2g4z

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[[Image:2g4z.jpg|left|200px]]
[[Image:2g4z.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2g4z |SIZE=350|CAPTION= <scene name='initialview01'>2g4z</scene>, resolution 1.98&Aring;
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The line below this paragraph, containing "STRUCTURE_2g4z", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=ZN:ZINC+ION'>ZN</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Thermolysin Thermolysin], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.4.24.27 3.4.24.27] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2g4z| PDB=2g4z | SCENE= }}
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|RELATEDENTRY=[[2g4h|2G4H]], [[2g4i|2G4I]], [[2g4j|2G4J]], [[2g4k|2G4K]], [[2g4l|2G4L]], [[2g4m|2G4M]], [[2g4n|2G4N]], [[2g4o|2G4O]], [[2g4p|2G4P]], [[2g4q|2G4Q]], [[2g4r|2G4R]], [[2g4s|2G4S]], [[2g4t|2G4T]], [[2g4u|2G4U]], [[2g4v|2G4V]], [[2g4w|2G4W]], [[2g4x|2G4X]], [[2g4y|2G4Y]], [[2g51|2G51]], [[2g52|2G52]], [[2g55|2G55]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g4z FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g4z OCA], [http://www.ebi.ac.uk/pdbsum/2g4z PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g4z RCSB]</span>
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}}
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'''anomalous substructure of thermolysin'''
'''anomalous substructure of thermolysin'''
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[[Category: Mueller-Dieckmann, C.]]
[[Category: Mueller-Dieckmann, C.]]
[[Category: Weiss, M S.]]
[[Category: Weiss, M S.]]
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[[Category: anomalous substructure of thermolysin]]
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[[Category: Anomalous substructure of thermolysin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:41:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:11:54 2008''
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Revision as of 01:41, 4 May 2008

Template:STRUCTURE 2g4z

anomalous substructure of thermolysin


Overview

23 different crystal forms of 19 different biological macromolecules were examined with respect to their anomalously scattering substructures using diffraction data collected at a wavelength of 2.0 A (6.2 keV). In more than 90% of the cases the substructure was found to contain more than just the protein S atoms. The data presented suggest that chloride, sulfate, phosphate or metal ions from the buffer or even from the purification protocol are frequently bound to the protein molecule and that these ions are often overlooked, especially if they are not bound at full occupancy. Thus, in order to fully describe the macromolecule under study, it seems desirable that any structure determination be complemented with a long-wavelength data set.

About this Structure

2G4Z is a Single protein structure of sequence from Bacillus thermoproteolyticus. Full crystallographic information is available from OCA.

Reference

On the routine use of soft X-rays in macromolecular crystallography. Part IV. Efficient determination of anomalous substructures in biomacromolecules using longer X-ray wavelengths., Mueller-Dieckmann C, Panjikar S, Schmidt A, Mueller S, Kuper J, Geerlof A, Wilmanns M, Singh RK, Tucker PA, Weiss MS, Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):366-80. Epub 2007, Feb 21. PMID:17327674 Page seeded by OCA on Sun May 4 04:41:44 2008

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