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2g5q

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[[Image:2g5q.gif|left|200px]]
[[Image:2g5q.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2g5q |SIZE=350|CAPTION= <scene name='initialview01'>2g5q</scene>, resolution 2.700&Aring;
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The line below this paragraph, containing "STRUCTURE_2g5q", creates the "Structure Box" on the page.
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|SITE=
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|LIGAND= <scene name='pdbligand=A:ADENOSINE-5&#39;-MONOPHOSPHATE'>A</scene>, <scene name='pdbligand=AKN:(2R)-4-AMINO-N-{(1R,2S,3S,4R,5S)-5-AMINO-2-[(3-AMINO-3-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-4-[(6-AMINO-6-DEOXY-ALPHA-D-GLUCOPYRANOSYL)OXY]-3-HYDROXYCYCLOHEXYL}-2-HYDROXYBUTANAMIDE'>AKN</scene>, <scene name='pdbligand=C:CYTIDINE-5&#39;-MONOPHOSPHATE'>C</scene>, <scene name='pdbligand=G:GUANOSINE-5&#39;-MONOPHOSPHATE'>G</scene>, <scene name='pdbligand=U:URIDINE-5&#39;-MONOPHOSPHATE'>U</scene>
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|ACTIVITY=
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|GENE=
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{{STRUCTURE_2g5q| PDB=2g5q | SCENE= }}
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|RELATEDENTRY=[[2g5k|2G5K]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g5q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g5q OCA], [http://www.ebi.ac.uk/pdbsum/2g5q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g5q RCSB]</span>
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'''Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.'''
'''Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.'''
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==About this Structure==
==About this Structure==
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2G5Q is a [[Protein complex]] structure of sequences from [http://en.wikipedia.org/wiki/ ]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5Q OCA].
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Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2G5Q OCA].
==Reference==
==Reference==
Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16806634 16806634]
Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16806634 16806634]
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[[Category: Protein complex]]
 
[[Category: Francois, B.]]
[[Category: Francois, B.]]
[[Category: Kondo, J.]]
[[Category: Kondo, J.]]
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[[Category: Russell, R J.M.]]
[[Category: Russell, R J.M.]]
[[Category: Westhof, E.]]
[[Category: Westhof, E.]]
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[[Category: amikacin]]
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[[Category: Amikacin]]
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[[Category: aminoglycoside]]
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[[Category: Aminoglycoside]]
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[[Category: haba group]]
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[[Category: Haba group]]
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[[Category: ribosomal decoding site]]
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[[Category: Ribosomal decoding site]]
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[[Category: rna]]
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[[Category: Rna]]
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[[Category: x-ray analysis]]
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[[Category: X-ray analysis]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:43:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:12:11 2008''
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Revision as of 01:43, 4 May 2008

Template:STRUCTURE 2g5q

Crystal structures of the bacterial ribosomal decoding site complexed with amikacin.


Overview

Amikacin is the 4,6-linked aminoglycoside modified at position N1 of the 2-deoxystreptamine ring (ring II) by the L-haba group. In the present study, the crystal structure of a complex between oligonucleotide containing the bacterial ribosomal A site and amikacin has been solved at 2.7 A resolution. Amikacin specifically binds to the A site in practically the same way as its parent compound kanamycin. In addition, the L-haba group interacts with the upper side of the A site through two direct contacts, O2*...H-N4(C1496) and N4*-H...O6(G1497). The present crystal structure shows how the introduction of the L-haba group on ring II of aminoglycoside is an effective mutation for obtaining a higher affinity to the bacterial A site.

About this Structure

Full crystallographic information is available from OCA.

Reference

Crystal structure of the bacterial ribosomal decoding site complexed with amikacin containing the gamma-amino-alpha-hydroxybutyryl (haba) group., Kondo J, Francois B, Russell RJ, Murray JB, Westhof E, Biochimie. 2006 Aug;88(8):1027-31. Epub 2006 Jun 13. PMID:16806634 Page seeded by OCA on Sun May 4 04:43:06 2008

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