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2g9b

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[[Image:2g9b.gif|left|200px]]
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|GENE= Calb1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10116 Rattus norvegicus])
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|RELATEDENTRY=[[2f33|2F33]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2g9b FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2g9b OCA], [http://www.ebi.ac.uk/pdbsum/2g9b PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2g9b RCSB]</span>
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'''NMR solution structure of CA2+-loaded calbindin D28K'''
'''NMR solution structure of CA2+-loaded calbindin D28K'''
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[[Category: Thompson, R J.]]
[[Category: Thompson, R J.]]
[[Category: Venters, R A.]]
[[Category: Venters, R A.]]
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[[Category: ca2+-binding]]
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[[Category: Ca2+-binding]]
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[[Category: ef-hand]]
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[[Category: Ef-hand]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 04:50:42 2008''
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Revision as of 01:50, 4 May 2008

Image:2g9b.gif

Template:STRUCTURE 2g9b

NMR solution structure of CA2+-loaded calbindin D28K


Overview

Calbindin-D(28K) is a Ca2+-binding protein, performing roles as both a calcium buffer and calcium sensor. The NMR solution structure of Ca2+-loaded calbindin-D(28K) reveals a single, globular fold consisting of six distinct EF-hand subdomains, which coordinate Ca2+ in loops on EF1, EF3, EF4 and EF5. Target peptides from Ran-binding protein M and myo-inositol monophosphatase, along with a new target from procaspase-3, are shown to interact with the protein on a surface comprised of alpha5 (EF3), alpha8 (EF4) and the EF2-EF3 and EF4-EF5 loops. Fluorescence experiments reveal that calbindin-D(28K) adopts discrete hydrophobic states as it binds Ca2+. The structure, binding interface and hydrophobic characteristics of Ca2+-loaded calbindin-D(28K) provide the first detailed insights into how this essential protein may function. This structure is one of the largest high-resolution NMR structures and the largest monomeric EF-hand protein to be solved to date.

About this Structure

2G9B is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Structure, binding interface and hydrophobic transitions of Ca2+-loaded calbindin-D(28K)., Kojetin DJ, Venters RA, Kordys DR, Thompson RJ, Kumar R, Cavanagh J, Nat Struct Mol Biol. 2006 Jul;13(7):641-7. Epub 2006 Jun 25. PMID:16799559 Page seeded by OCA on Sun May 4 04:50:42 2008

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