2gaf
From Proteopedia
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'''Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)''' | '''Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)''' | ||
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[[Category: Protein complex]] | [[Category: Protein complex]] | ||
[[Category: Vaccinia virus]] | [[Category: Vaccinia virus]] | ||
| - | [[Category: mRNA (nucleoside-2'-O-)-methyltransferase]] | ||
[[Category: Bowman, B R.]] | [[Category: Bowman, B R.]] | ||
[[Category: Gershon, P D.]] | [[Category: Gershon, P D.]] | ||
[[Category: Moure, C M.]] | [[Category: Moure, C M.]] | ||
[[Category: Quiocho, F A.]] | [[Category: Quiocho, F A.]] | ||
| - | [[Category: | + | [[Category: Heterodimer]] |
| - | [[Category: | + | [[Category: Nucleotidyltransferase]] |
| - | [[Category: | + | [[Category: Polyadenylate polymerase]] |
| - | [[Category: | + | [[Category: Pox virus]] |
| - | [[Category: | + | [[Category: Processivity]] |
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Revision as of 01:53, 4 May 2008
Crystal Structure of the Vaccinia Polyadenylate Polymerase Heterodimer (apo form)
Overview
Polyadenylation of mRNAs in poxviruses, crucial for virion maturation, is carried out by a poly(A) polymerase heterodimer composed of a catalytic component, VP55, and a processivity factor, VP39. The ATP-gamma-S bound and unbound crystal structures of the vaccinia polymerase reveal an unusual architecture for VP55 that comprises of N-terminal, central or catalytic, and C-terminal domains with different topologies and that differs from many polymerases, including the eukaryotic poly(A) polymerases. Residues in the active site of VP55, located between the catalytic and C-terminal domains, make specific interactions with the adenine of the ATP analog, establishing the molecular basis of ATP recognition. VP55's concave surface docks the globular VP39. A model for RNA primer binding that involves all three VP55 domains and VP39 is proposed. The model supports biochemical evidence that VP39 functions as a processivity factor by partially enclosing the RNA primer at the heterodimer interface.
About this Structure
2GAF is a Protein complex structure of sequences from Vaccinia virus. Full crystallographic information is available from OCA.
Reference
Crystal structures of the vaccinia virus polyadenylate polymerase heterodimer: insights into ATP selectivity and processivity., Moure CM, Bowman BR, Gershon PD, Quiocho FA, Mol Cell. 2006 May 5;22(3):339-49. PMID:16678106 Page seeded by OCA on Sun May 4 04:53:00 2008
