2gjr

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[[Image:2gjr.jpg|left|200px]]
[[Image:2gjr.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2gjr |SIZE=350|CAPTION= <scene name='initialview01'>2gjr</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_2gjr", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ACT:ACETATE+ION'>ACT</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Alpha-amylase Alpha-amylase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.2.1.1 3.2.1.1] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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|DOMAIN=
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{{STRUCTURE_2gjr| PDB=2gjr | SCENE= }}
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|RELATEDENTRY=[[1w9x|1W9X]], [[2gjp|2GJP]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gjr FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gjr OCA], [http://www.ebi.ac.uk/pdbsum/2gjr PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gjr RCSB]</span>
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}}
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'''Structure of bacillus halmapalus alpha-amylase without any substrate analogues'''
'''Structure of bacillus halmapalus alpha-amylase without any substrate analogues'''
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[[Category: Kaasgaard, S G.]]
[[Category: Kaasgaard, S G.]]
[[Category: Lyhne-Iversen, L.]]
[[Category: Lyhne-Iversen, L.]]
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[[Category: alpha-amylase]]
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[[Category: Alpha-amylase]]
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[[Category: bacillus halmapalus]]
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[[Category: Bacillus halmapalus]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:11:07 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:17:35 2008''
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Revision as of 02:11, 4 May 2008

Image:2gjr.jpg

Template:STRUCTURE 2gjr

Structure of bacillus halmapalus alpha-amylase without any substrate analogues


Overview

Recombinant Bacillus halmapalus alpha-amylase (BHA) was studied in two different crystal forms. The first crystal form was obtained by crystallization of BHA at room temperature in the presence of acarbose and maltose; data were collected at cryogenic temperature to a resolution of 1.9 A. It was found that the crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 47.0, b = 73.5, c = 151.1 A. A maltose molecule was observed and found to bind to BHA and previous reports of the binding of a nonasaccharide were confirmed. The second crystal form was obtained by pH-induced crystallization of BHA in a MES-HEPES-boric acid buffer (MHB buffer) at 303 K; the solubility of BHA in MHB has a retrograde temperature dependency and crystallization of BHA was only possible by raising the temperature to at least 298 K. Data were collected at cryogenic temperature to a resolution of 2.0 A. The crystal belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 38.6, b = 59.0, c = 209.8 A. The structure was solved using molecular replacement. The maltose-binding site is described and the two structures are compared. No significant changes were seen in the structure upon binding of the substrates.

About this Structure

2GJR is a Single protein structure of sequence from Bacillus halmapalus. Full crystallographic information is available from OCA.

Reference

Structure of Bacillus halmapalus alpha-amylase crystallized with and without the substrate analogue acarbose and maltose., Lyhne-Iversen L, Hobley TJ, Kaasgaard SG, Harris P, Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Sep 1;62(Pt, 9):849-54. Epub 2006 Aug 26. PMID:16946462 Page seeded by OCA on Sun May 4 05:11:07 2008

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