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2gmi
From Proteopedia
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[[Image:2gmi.jpg|left|200px]] | [[Image:2gmi.jpg|left|200px]] | ||
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'''Mms2/Ubc13~Ubiquitin''' | '''Mms2/Ubc13~Ubiquitin''' | ||
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[[Category: Pickart, C M.]] | [[Category: Pickart, C M.]] | ||
[[Category: Wolberger, C.]] | [[Category: Wolberger, C.]] | ||
| - | [[Category: | + | [[Category: E2]] |
| - | [[Category: | + | [[Category: Mms2]] |
| - | [[Category: | + | [[Category: Ubc13]] |
| - | [[Category: | + | [[Category: Ubc13 ubiquitin covalent complex]] |
| - | [[Category: | + | [[Category: Ubiquitin]] |
| - | [[Category: | + | [[Category: Uev]] |
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Revision as of 02:16, 4 May 2008
Mms2/Ubc13~Ubiquitin
Overview
Lys63-linked polyubiquitin chains participate in nonproteolytic signaling pathways, including regulation of DNA damage tolerance and NF-kappaB activation. E2 enzymes bound to ubiquitin E2 variants (UEV) are vital in these pathways, synthesizing Lys63-linked polyubiquitin chains, but how these complexes achieve specificity for a particular lysine linkage has been unclear. We have determined the crystal structure of an Mms2-Ubc13-ubiquitin (UEV-E2-Ub) covalent intermediate with donor ubiquitin linked to the active site residue of Ubc13. In the structure, the unexpected binding of a donor ubiquitin of one Mms2-Ubc13-Ub complex to the acceptor-binding site of Mms2-Ubc13 in an adjacent complex allows us to visualize at atomic resolution the molecular determinants of acceptor-ubiquitin binding. The structure reveals the key role of Mms2 in allowing selective insertion of Lys63 into the Ubc13 active site and suggests a molecular model for polyubiquitin chain elongation.
About this Structure
2GMI is a Protein complex structure of sequences from Homo sapiens and Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Mms2-Ubc13 covalently bound to ubiquitin reveals the structural basis of linkage-specific polyubiquitin chain formation., Eddins MJ, Carlile CM, Gomez KM, Pickart CM, Wolberger C, Nat Struct Mol Biol. 2006 Oct;13(10):915-20. Epub 2006 Sep 17. PMID:16980971 Page seeded by OCA on Sun May 4 05:16:20 2008
