Vpr protein

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 3: Line 3:
== Function ==
== Function ==
'''Vpr protein''' (Vpr) or '''viral protein R''' is a 96 amino acid protein which encoded by the human Immunodeficiency virus type 1 ([[HIV-1]]). HIV-1 genome includes nine genes, six of them are accessory proteins, including ''vpr''. Vpr has several roles in the progression of acquired immunodeficiency syndrome ([https://en.wikipedia.org/wiki/AIDS AIDS]) disease, including regulation the HIV-1 pre-integration complex ([https://en.wikipedia.org/wiki/Pre-integration_complex PIC]) nuclear import and virus replication in non-dividing macrophages<ref name='Morellet2003'>PMID:12614620</ref>. Moreover, Vpr prevents mitosis of dividing infect cells by blockade at G2 phase, when the viral promoter is more active, and induced apoptosis of these cells in G1 and M phase. Both the activities of cell cycle arrest and induce apoptosis has proven to be independence by mutations that can cause only one of these affects<ref name='Gonzalez2017'>PMID:28075409</ref>.
'''Vpr protein''' (Vpr) or '''viral protein R''' is a 96 amino acid protein which encoded by the human Immunodeficiency virus type 1 ([[HIV-1]]). HIV-1 genome includes nine genes, six of them are accessory proteins, including ''vpr''. Vpr has several roles in the progression of acquired immunodeficiency syndrome ([https://en.wikipedia.org/wiki/AIDS AIDS]) disease, including regulation the HIV-1 pre-integration complex ([https://en.wikipedia.org/wiki/Pre-integration_complex PIC]) nuclear import and virus replication in non-dividing macrophages<ref name='Morellet2003'>PMID:12614620</ref>. Moreover, Vpr prevents mitosis of dividing infect cells by blockade at G2 phase, when the viral promoter is more active, and induced apoptosis of these cells in G1 and M phase. Both the activities of cell cycle arrest and induce apoptosis has proven to be independence by mutations that can cause only one of these affects<ref name='Gonzalez2017'>PMID:28075409</ref>.
 +
 +
=== Vpr induce cell cycle arrest by recruits cellular targets for degradation<ref>PMID:27571178</ref> ===
 +
One phenotype of Vpr expression is the induction of cell cycle arrest in G2 phase. This arrest executes by engaging of DNA damage–binding protein 1 (DDB1) and CUL4A-associated factor 1 (DCAF1) to Vpr. DCAF1 is a substrate receptor of the Cullin4–RING E3 ubiquitin ligase (CRL4) of the host ubiquitin–proteasome-mediated protein degradation pathway. Mutations in Vpr that abolish its interaction with DCAF1, or silencing of DCAF1, eliminate cell-cycle arrest.
== Disease ==
== Disease ==

Revision as of 00:11, 8 April 2018

HIV-1 Vpr protein (cyan) complex with Human DNA damage-binding protein (grey), VPRBP (pink) and Uracyl-DNA glycosylase (yellow) (PDB code 5jk7)

Drag the structure with the mouse to rotate

3D Structures of Vpr protein

Updated on 08-April-2018

1m8l – Vpr - NMR - HIV-1
1esx - synthetic Vpr - NMR - HIV-1
5jk7 – Vpr + DDB1 + DCAF-1 + UNG2 – X-ray solution - HIV-1
1x9v – Dimeric structure of the Vpr C-terminal domain - NMR
1vpc - C-terminal domain of Vpr - NMR - HIV-1
1fi0 - Vpr residues 13-33 in micelles - NMR - HIV-1
1bde - NMR solution of Vpr peptides connected to cell cycle arrest and nuclear provirus transfer
5b56 - Importin subunit alpha-1 + Vpr C-terminal domain - crystallographic analysis
1kzs, 1kzt, 1kzv - Vpr residues 34-51 - NMR - HIV-1
1dsj - Vpr residues 50-75 - NMR - HIV-1
1ceu - Vpr N-terminal domain - NMR - HIV-1
1dsk - Vpr residues 59-86 - NMR - HIV-1


References

  1. ↑ 1.0 1.1 Morellet N, Bouaziz S, Petitjean P, Roques BP. NMR structure of the HIV-1 regulatory protein VPR. J Mol Biol. 2003 Mar 14;327(1):215-27. PMID:12614620
  2. ↑ 2.0 2.1 Gonzalez ME. The HIV-1 Vpr Protein: A Multifaceted Target for Therapeutic Intervention. Int J Mol Sci. 2017 Jan 10;18(1). pii: ijms18010126. doi: 10.3390/ijms18010126. PMID:28075409 doi:http://dx.doi.org/10.3390/ijms18010126
  3. ↑ Wu Y, Zhou X, Barnes CO, DeLucia M, Cohen AE, Gronenborn AM, Ahn J, Calero G. The DDB1-DCAF1-Vpr-UNG2 crystal structure reveals how HIV-1 Vpr steers human UNG2 toward destruction. Nat Struct Mol Biol. 2016 Aug 29. doi: 10.1038/nsmb.3284. PMID:27571178 doi:http://dx.doi.org/10.1038/nsmb.3284
  4. ↑ 4.0 4.1 Emerman M. HIV-1, Vpr and the cell cycle. Curr Biol. 1996 Sep 1;6(9):1096-103. PMID:8805364

Proteopedia Page Contributors and Editors (what is this?)

Elia Shlush, Michal Harel

Retrieved from "http://52.214.119.220/wiki/index.php/Vpr_protein"
Personal tools