2grq

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[[Image:2grq.gif|left|200px]]
[[Image:2grq.gif|left|200px]]
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{{Structure
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|PDB= 2grq |SIZE=350|CAPTION= <scene name='initialview01'>2grq</scene>, resolution 1.700&Aring;
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The line below this paragraph, containing "STRUCTURE_2grq", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND=
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Ubiquitin--protein_ligase Ubiquitin--protein ligase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=6.3.2.19 6.3.2.19] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= UBE2I, UBC9, UBCE9 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens]), RANGAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 Homo sapiens])
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|DOMAIN=
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{{STRUCTURE_2grq| PDB=2grq | SCENE= }}
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|RELATEDENTRY=[[2grn|2GRN]], [[2gro|2GRO]], [[2grp|2GRP]], [[2grr|2GRR]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2grq FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2grq OCA], [http://www.ebi.ac.uk/pdbsum/2grq PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2grq RCSB]</span>
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'''Crystal Structure of human RanGAP1-Ubc9-D127A'''
'''Crystal Structure of human RanGAP1-Ubc9-D127A'''
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[[Category: Lima, C D.]]
[[Category: Lima, C D.]]
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[[Category: conjugation]]
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[[Category: Conjugation]]
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[[Category: small ubiquitin like modifer]]
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[[Category: Small ubiquitin like modifer]]
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[[Category: smt3]]
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[[Category: Smt3]]
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[[Category: ubiquitin]]
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[[Category: Ubiquitin]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:26:39 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:20:38 2008''
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Revision as of 02:26, 4 May 2008

Image:2grq.gif

Template:STRUCTURE 2grq

Crystal Structure of human RanGAP1-Ubc9-D127A


Overview

E2 conjugating proteins that transfer ubiquitin and ubiquitin-like modifiers to substrate lysine residues must first activate the lysine nucleophile for conjugation. Genetic complementation revealed three side chains of the E2 Ubc9 that were crucial for normal growth. Kinetic analysis revealed modest binding defects but substantially lowered catalytic rates for these mutant alleles with respect to wild-type Ubc9. X-ray structures for wild-type and mutant human Ubc9-RanGAP1 complexes showed partial loss of contacts to the substrate lysine in mutant complexes. Computational analysis predicted pK perturbations for the substrate lysine, and Ubc9 mutations weakened pK suppression through improper side chain coordination. Biochemical studies with p53, RanGAP1 and the Nup358/RanBP2 E3 were used to determine rate constants and pK values, confirming both structural and computational predictions. It seems that Ubc9 uses an indirect mechanism to activate lysine for conjugation that may be conserved among E2 family members.

About this Structure

2GRQ is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Lysine activation and functional analysis of E2-mediated conjugation in the SUMO pathway., Yunus AA, Lima CD, Nat Struct Mol Biol. 2006 Jun;13(6):491-9. Epub 2006 May 28. PMID:16732283 Page seeded by OCA on Sun May 4 05:26:39 2008

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