2gsz

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[[Image:2gsz.jpg|left|200px]]
[[Image:2gsz.jpg|left|200px]]
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{{Structure
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|PDB= 2gsz |SIZE=350|CAPTION= <scene name='initialview01'>2gsz</scene>, resolution 4.20&Aring;
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The line below this paragraph, containing "STRUCTURE_2gsz", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ADP:ADENOSINE-5&#39;-DIPHOSPHATE'>ADP</scene>, <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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|GENE= pilT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 Aquifex aeolicus])
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|DOMAIN=
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{{STRUCTURE_2gsz| PDB=2gsz | SCENE= }}
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|RELATEDENTRY=[[2eyu|2EYU]], [[2ewv|2EWV]], [[2eww|2EWW]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2gsz FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2gsz OCA], [http://www.ebi.ac.uk/pdbsum/2gsz PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2gsz RCSB]</span>
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'''Structure of A. aeolicus PilT with 6 monomers per asymmetric unit'''
'''Structure of A. aeolicus PilT with 6 monomers per asymmetric unit'''
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[[Category: Forest, K T.]]
[[Category: Forest, K T.]]
[[Category: Satyshur, K A.]]
[[Category: Satyshur, K A.]]
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[[Category: atpase]]
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[[Category: Atpase]]
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[[Category: domain motion]]
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[[Category: Domain motion]]
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[[Category: p-loop]]
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[[Category: P-loop]]
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[[Category: pa]]
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[[Category: Pa]]
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[[Category: reca]]
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[[Category: Reca]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:29:36 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:21:14 2008''
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Revision as of 02:29, 4 May 2008

Image:2gsz.jpg

Template:STRUCTURE 2gsz

Structure of A. aeolicus PilT with 6 monomers per asymmetric unit


Overview

PilT is a hexameric ATPase required for bacterial type IV pilus retraction and surface motility. Crystal structures of ADP- and ATP-bound Aquifex aeolicus PilT at 2.8 and 3.2 A resolution show N-terminal PAS-like and C-terminal RecA-like ATPase domains followed by a set of short C-terminal helices. The hexamer is formed by extensive polar subunit interactions between the ATPase core of one monomer and the N-terminal domain of the next. An additional structure captures a nonsymmetric PilT hexamer in which approach of invariant arginines from two subunits to the bound nucleotide forms an enzymatically competent active site. A panel of pilT mutations highlights the importance of the arginines, the PAS-like domain, the polar subunit interface, and the C-terminal helices for retraction. We present a model for ATP binding leading to dramatic PilT domain motions, engagement of the arginine wire, and subunit communication in this hexameric motor. Our conclusions apply to the entire type II/IV secretion ATPase family.

About this Structure

2GSZ is a Single protein structure of sequence from Aquifex aeolicus. Full crystallographic information is available from OCA.

Reference

Crystal structures of the pilus retraction motor PilT suggest large domain movements and subunit cooperation drive motility., Satyshur KA, Worzalla GA, Meyer LS, Heiniger EK, Aukema KG, Misic AM, Forest KT, Structure. 2007 Mar;15(3):363-76. PMID:17355871 Page seeded by OCA on Sun May 4 05:29:36 2008

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