This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2gv5
From Proteopedia
| Line 1: | Line 1: | ||
[[Image:2gv5.gif|left|200px]] | [[Image:2gv5.gif|left|200px]] | ||
| - | + | <!-- | |
| - | + | The line below this paragraph, containing "STRUCTURE_2gv5", creates the "Structure Box" on the page. | |
| - | + | You may change the PDB parameter (which sets the PDB file loaded into the applet) | |
| - | + | or the SCENE parameter (which sets the initial scene displayed when the page is loaded), | |
| - | + | or leave the SCENE parameter empty for the default display. | |
| - | + | --> | |
| - | + | {{STRUCTURE_2gv5| PDB=2gv5 | SCENE= }} | |
| - | | | + | |
| - | | | + | |
| - | }} | + | |
'''crystal structure of Sfi1p/Cdc31p complex''' | '''crystal structure of Sfi1p/Cdc31p complex''' | ||
| Line 31: | Line 28: | ||
[[Category: Sandercock, A M.]] | [[Category: Sandercock, A M.]] | ||
[[Category: Williams, R L.]] | [[Category: Williams, R L.]] | ||
| - | [[Category: | + | [[Category: Cdc31p]] |
| - | [[Category: | + | [[Category: Centrin]] |
| - | [[Category: | + | [[Category: Centrosome]] |
| - | [[Category: | + | [[Category: Sfi1p]] |
| - | [[Category: | + | [[Category: Spindle pole body]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:34:36 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 02:34, 4 May 2008
crystal structure of Sfi1p/Cdc31p complex
Overview
Centrins are calmodulin-like proteins present in centrosomes and yeast spindle pole bodies (SPBs) and have essential functions in their duplication. The Saccharomyces cerevisiae centrin, Cdc31p, binds Sfi1p on multiple conserved repeats; both proteins localize to the SPB half-bridge, where the new SPB is assembled. The crystal structures of Sfi1p-centrin complexes containing several repeats show Sfi1p as an alpha helix with centrins wrapped around each repeat and similar centrin-centrin contacts between each repeat. Electron microscopy (EM) shadowing of an Sfi1p-centrin complex with 15 Sfi1 repeats and 15 centrins bound showed filaments 60 nm long, compatible with all the Sfi1 repeats as a continuous alpha helix. Immuno-EM localization of the Sfi1p N and C termini showed Sfi1p-centrin filaments spanning the length of the half-bridge with the Sfi1p N terminus at the SPB. This suggests a model for SPB duplication where the half-bridge doubles in length by association of the Sfi1p C termini, thereby providing a new Sfi1p N terminus to initiate SPB assembly.
About this Structure
2GV5 is a Protein complex structure of sequences from Saccharomyces cerevisiae. Full crystallographic information is available from OCA.
Reference
Structural role of Sfi1p-centrin filaments in budding yeast spindle pole body duplication., Li S, Sandercock AM, Conduit P, Robinson CV, Williams RL, Kilmartin JV, J Cell Biol. 2006 Jun 19;173(6):867-77. PMID:16785321 Page seeded by OCA on Sun May 4 05:34:36 2008
