This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.

Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.

1whi

From Proteopedia

(Difference between revisions)

Jump to: navigation, search

Revision as of 07:46, 30 December 2020

RIBOSOMAL PROTEIN L14

Structural highlights

1whi is a 1 chain structure with sequence from Atcc 12980. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	BACILLUS STEAROTHERMOPHILUS (ATCC 12980)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RL14_GEOSE] Forms part of two intersubunit bridges in the 70S ribosome (By similarity). Binds to 23S rRNA.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

BACKGROUND: Detailed structural information on ribosomal proteins has increased our understanding of the structure, function and evolution of the ribosome. L14 is one of the most conserved ribosomal proteins and appears to have a central role in the ribonucleoprotein complex. Studies have indicated that L14 occupies a central location between the peptidyl transferase and GTPase regions of the large ribosomal subunit. RESULTS: The crystal structure of L14 from Bacillus stearothermophilus has been solved using a combination of isomorphous replacement and multiwavelength anomalous dispersion (MAD) methods. The structure comprises a five-stranded beta-barrel, a C-terminal loop region that contains two small alpha-helices, and a beta-ribbon that projects from the beta-barrel. An analysis of the structure and the conserved amino acids reveals three surface patches that probably mediate L14-RNA and L14-protein interactions within the ribosome. CONCLUSIONS: The accepted role of ribosomal proteins is to promote the folding and stabilization of ribosomal RNA. The L14 structure is consistent with this notion, and it suggests that the RNA binds in two sites. One RNA-binding site appears to recognize a distinct region of ribosomal RNA during particle assembly. The second site is smaller and may become occupied during the later compaction of the RNA. The surface hydrophobic patch is a likely site of protein-protein interaction, possibly with L19.

The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus.,Davies C, White SW, Ramakrishnan V Structure. 1996 Jan 15;4(1):55-66. PMID:8805509^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Davies C, White SW, Ramakrishnan V. The crystal structure of ribosomal protein L14 reveals an important organizational component of the translational apparatus. Structure. 1996 Jan 15;4(1):55-66. PMID:8805509

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1whi"

@@ Line 1: / Line 1: @@
 ==RIBOSOMAL PROTEIN L14==
-<StructureSection load='1whi' size='340' side='right' caption='[[1whi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
+<StructureSection load='1whi' size='340' side='right'caption='[[1whi]], [[Resolution|resolution]] 1.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1whi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WHI OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1WHI FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1whi]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Atcc_12980 Atcc 12980]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1WHI OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1WHI FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">BACILLUS STEAROTHERMOPHILUS ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=1422 ATCC 12980])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1whi OCA], [http://pdbe.org/1whi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1whi RCSB], [http://www.ebi.ac.uk/pdbsum/1whi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1whi ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1whi FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1whi OCA], [http://pdbe.org/1whi PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1whi RCSB], [http://www.ebi.ac.uk/pdbsum/1whi PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1whi ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 36: / Line 36: @@
 </StructureSection>
 [[Category: Atcc 12980]]
+[[Category: Large Structures]]
 [[Category: Davies, C]]
 [[Category: Ramakrishnan, V]]

1whi

From Proteopedia

Revision as of 07:46, 30 December 2020

RIBOSOMAL PROTEIN L14

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox