2gzw
From Proteopedia
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'''Crystal structure of the E.coli CRP-cAMP complex''' | '''Crystal structure of the E.coli CRP-cAMP complex''' | ||
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[[Category: Tanaka, T.]] | [[Category: Tanaka, T.]] | ||
[[Category: Yokoyama, S.]] | [[Category: Yokoyama, S.]] | ||
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| - | [[Category: | + | [[Category: Riken structural genomics/proteomics initiative]] |
| - | [[Category: | + | [[Category: Rsgi]] |
| - | [[Category: | + | [[Category: Structural genomic]] |
| - | [[Category: | + | [[Category: Transcription]] |
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Revision as of 02:42, 4 May 2008
Crystal structure of the E.coli CRP-cAMP complex
Overview
The structure of a dimer of the Escherichia coli catabolite gene activator protein has been refined at 2.5 A resolution to a crystallographic R-factor of 20.7% starting with coordinates fitted to the map at 2.9 A resolution. The two subunits are in different conformations and each contains one bound molecule of the allosteric activator, cyclic AMP. The amino-terminal domain is linked to the smaller carboxy-terminal domain by a nine-residue hinge region that exists in different conformations in the two subunits, giving rise to approximately a 30 degree rotation between the positions of the small domains relative to the larger domains. The amino-terminal domain contains an antiparallel beta-roll structure in which the interstrand hydrogen bonding is well-determined. The beta-roll can be described as a long antiparallel beta-ribbon that folds into a right-handed supercoil and forms part of the cyclic AMP binding site. Each cyclic AMP molecule is in an anti conformation and has ionic and hydrogen bond interactions with both subunits.
About this Structure
2GZW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
Structure of a complex of catabolite gene activator protein and cyclic AMP refined at 2.5 A resolution., Weber IT, Steitz TA, J Mol Biol. 1987 Nov 20;198(2):311-26. PMID:2828639 Page seeded by OCA on Sun May 4 05:42:06 2008
Categories: Escherichia coli | Single protein | Kumarevel, T S. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Shinkai, A. | Tanaka, T. | Yokoyama, S. | Camp binding protein | Crp | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Structural genomic | Transcription
