2h1k

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[[Image:2h1k.gif|left|200px]]
[[Image:2h1k.gif|left|200px]]
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{{Structure
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|PDB= 2h1k |SIZE=350|CAPTION= <scene name='initialview01'>2h1k</scene>, resolution 2.42&Aring;
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The line below this paragraph, containing "STRUCTURE_2h1k", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= IPF1, PDX1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10036 Mesocricetus auratus])
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|DOMAIN=
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{{STRUCTURE_2h1k| PDB=2h1k | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2h1k FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2h1k OCA], [http://www.ebi.ac.uk/pdbsum/2h1k PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2h1k RCSB]</span>
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'''Crystal structure of the Pdx1 homeodomain in complex with DNA'''
'''Crystal structure of the Pdx1 homeodomain in complex with DNA'''
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[[Category: Longo, A.]]
[[Category: Longo, A.]]
[[Category: Rose, R B.]]
[[Category: Rose, R B.]]
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[[Category: protein-dna complex]]
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[[Category: Protein-dna complex]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:45:42 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:24:10 2008''
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Revision as of 02:45, 4 May 2008

Image:2h1k.gif


PDB ID 2h1k

Drag the structure with the mouse to rotate
2h1k, resolution 2.42Å ()
Gene: IPF1, PDX1 (Mesocricetus auratus)
Resources: FirstGlance, OCA, RCSB, PDBsum
Coordinates: save as pdb, mmCIF, xml



Crystal structure of the Pdx1 homeodomain in complex with DNA


Overview

Pancreatic and duodenal homeobox 1 (Pdx1) is a homeodomain transcription factor belonging to the ParaHox family. Pdx1 plays an essential role in pancreatic endocrine and exocrine cell development and maintenance of adult islet beta-cell function. Mutations in the human pdx1 gene are linked to an early onset form of non-insulin-dependent diabetes mellitus, MODY-4. We demonstrate that the homeodomain reproduces the binding specificity of the full-length protein. We report the 2.4 A resolution crystal structure of the homeodomain bound to a target DNA. The two Pdx1/DNA complexes in the asymmetric unit display conformational differences: in the DNA curvature, the orientation of the homeodomain in the major groove, and the order of the N-terminal arm. Comparing the two complexes indicates invariant protein-DNA contacts, and variant contacts that are unique to each binding orientation. An induced fit model is proposed that depends on the DNA conformation and provides a mechanism for nonlocal contributions to binding specificity.

About this Structure

2H1K is a Single protein structure of sequence from Mesocricetus auratus. Full crystallographic information is available from OCA.

Reference

Structural basis for induced fit mechanisms in DNA recognition by the Pdx1 homeodomain., Longo A, Guanga GP, Rose RB, Biochemistry. 2007 Mar 20;46(11):2948-57. Epub 2007 Feb 23. PMID:17315980 Page seeded by OCA on Sun May 4 05:45:42 2008

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