5xes
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==TK9 NMR structure in SDS micelle== | ==TK9 NMR structure in SDS micelle== | ||
| - | <StructureSection load='5xes' size='340' side='right' caption='[[5xes]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> | + | <StructureSection load='5xes' size='340' side='right'caption='[[5xes]], [[NMR_Ensembles_of_Models | 20 NMR models]]' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5xes]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XES FirstGlance]. <br> | <table><tr><td colspan='2'>[[5xes]] is a 1 chain structure. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XES OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XES FirstGlance]. <br> | ||
| Line 7: | Line 7: | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xes OCA], [http://pdbe.org/5xes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xes RCSB], [http://www.ebi.ac.uk/pdbsum/5xes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xes ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xes FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xes OCA], [http://pdbe.org/5xes PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xes RCSB], [http://www.ebi.ac.uk/pdbsum/5xes PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xes ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | In recent years, several studies based on the interaction of self-assembling short peptides derived from viroporins with model membranes, have improved our understanding of the molecular mechanism of corona virus (CoV) infection under physiological conditions. In this study, we have characterized the mechanism of membrane interaction of a short, 9-residue peptide TK9 (T(55)VYVYSRVK(63)) that had been derived from the carboxyl terminal of the Severe Acute Respiratory Syndrome (SARS) corona virus (SARS CoV) envelope (E) protein. The peptide has been studied for its physical changes in the presence of both zwitterionic DPC and negatively charged SDS model membrane micelles, respectively, with the help of a battery of biophysical techniques including two-dimensional solution state NMR spectroscopy. Interestingly, in both micellar environments, TK9 adopted an alpha helical conformation; however, the helical propensities were much higher in the case of DPC compared to those of SDS micelle, suggesting that TK9 has more specificity towards eukaryotic cell membrane than the bacterial cell membrane. The orientation of the peptide TK9 also varies in the different micellar environments. The peptide's affinity was further manifested by its pronounced membrane disruption ability towards the mammalian compared to the bacterial membrane mimic. Collectively, the in-depth structural information on the interaction of TK9 with different membrane environments explains the host specificity and membrane orientation owing to subsequent membrane disruption implicated in the viral pathogenesis. | ||
| + | |||
| + | Structural insights of a self-assembling 9-residue peptide from the C-terminal tail of the SARS corona virus E-protein in DPC and SDS micelles: A combined high and low resolution spectroscopic study.,Ghosh A, Bhattacharyya D, Bhunia A Biochim Biophys Acta Biomembr. 2018 Feb;1860(2):335-346. doi:, 10.1016/j.bbamem.2017.10.015. Epub 2017 Oct 14. PMID:29038024<ref>PMID:29038024</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5xes" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
| + | [[Category: Large Structures]] | ||
[[Category: Bhunia, A]] | [[Category: Bhunia, A]] | ||
[[Category: Ghosh, A]] | [[Category: Ghosh, A]] | ||
[[Category: Protein fibril]] | [[Category: Protein fibril]] | ||
Revision as of 09:26, 30 October 2019
TK9 NMR structure in SDS micelle
| |||||||||||
