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5z67

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Structure of the recombination mediator protein RecF in RecFOR pathway

Structural highlights

5z67 is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RECF_CALS4] The RecF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP.

Publication Abstract from PubMed

RecF is a principal member of the RecF pathway. It interacts with RecO and RecR to initiate homologous recombination by loading RecA recombinases on single-stranded DNA and displacing single-stranded DNA-binding proteins. As an ATP-binding cassette ATPase, RecF exhibits ATP-dependent dimerization and structural homology with Rad50 and SMC proteins. However, the mechanism and action pattern of RecF ATP-dependent dimerization remains unclear. Here, We determined three crystal structures of TTERecF, TTERecF-ATP and TTERecF-ATPS from Thermoanaerobacter tengcongensis that reveal a novel ATP-driven RecF dimerization. RecF contains a positively charged tunnel on its dimer interface that is essential to ATP binding. Our structural and biochemical data indicate that the Walker A motif serves as a switch and plays a key role in ATP binding and RecF dimerization. Furthermore, Biolayer interferometry assay results showed that the TTERecF interacted with ATP and formed a dimer, displaying a higher affinity for DNA than that of the TTERecF monomer. Overall, our results provide a solid structural basis for understanding the process of RecF binding with ATP and the functional mechanism of ATP-dependent RecF dimerization.

ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair.,Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX Sci Rep. 2018 Feb 1;8(1):2127. doi: 10.1038/s41598-018-20557-0. PMID:29391496^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Tang Q, Liu YP, Shan HH, Tian LF, Zhang JZ, Yan XX. ATP-dependent conformational change in ABC-ATPase RecF serves as a switch in DNA repair. Sci Rep. 2018 Feb 1;8(1):2127. doi: 10.1038/s41598-018-20557-0. PMID:29391496 doi:http://dx.doi.org/10.1038/s41598-018-20557-0

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5z67"

@@ Line 1: / Line 1: @@
 ==Structure of the recombination mediator protein RecF in RecFOR pathway==
-<StructureSection load='5z67' size='340' side='right' caption='[[5z67]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
+<StructureSection load='5z67' size='340' side='right'caption='[[5z67]], [[Resolution|resolution]] 2.20&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5z67]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z67 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5Z67 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5z67]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5Z67 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5Z67 FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5z67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z67 OCA], [http://pdbe.org/5z67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z67 RCSB], [http://www.ebi.ac.uk/pdbsum/5z67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z67 ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5z67 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5z67 OCA], [http://pdbe.org/5z67 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5z67 RCSB], [http://www.ebi.ac.uk/pdbsum/5z67 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5z67 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 21: / Line 21: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Liu, Y P]]
 [[Category: Tang, Q]]

5z67

From Proteopedia

Current revision

Structure of the recombination mediator protein RecF in RecFOR pathway

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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