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| | ==Crystal structure of the Prp38-MFAP1 complex of Homo sapiens== | | ==Crystal structure of the Prp38-MFAP1 complex of Homo sapiens== |
| - | <StructureSection load='5f5s' size='340' side='right' caption='[[5f5s]], [[Resolution|resolution]] 2.40Å' scene=''> | + | <StructureSection load='5f5s' size='340' side='right'caption='[[5f5s]], [[Resolution|resolution]] 2.40Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5f5s]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5S OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5F5S FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5f5s]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5F5S OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5F5S FirstGlance]. <br> |
| - | </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">PRPF38A ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN]), MFAP1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.4Å</td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5f5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5s OCA], [http://pdbe.org/5f5s PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5f5s RCSB], [http://www.ebi.ac.uk/pdbsum/5f5s PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5s ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5f5s FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5f5s OCA], [https://pdbe.org/5f5s PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5f5s RCSB], [https://www.ebi.ac.uk/pdbsum/5f5s PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5f5s ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/PR38A_HUMAN PR38A_HUMAN]] May be required for pre-mRNA splicing. [[http://www.uniprot.org/uniprot/MFAP1_HUMAN MFAP1_HUMAN]] Component of the elastin-associated microfibrils. | + | [https://www.uniprot.org/uniprot/PR38A_HUMAN PR38A_HUMAN] May be required for pre-mRNA splicing. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | ==See Also== | | ==See Also== |
| - | *[[Pre-mRNA-splicing factor|Pre-mRNA-splicing factor]] | + | *[[Pre-mRNA splicing factors 3D structures|Pre-mRNA splicing factors 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Human]] | + | [[Category: Homo sapiens]] |
| - | [[Category: Ulrich, A K.C]] | + | [[Category: Large Structures]] |
| - | [[Category: Wahl, M C]] | + | [[Category: Ulrich AKC]] |
| - | [[Category: B-specific protein]] | + | [[Category: Wahl MC]] |
| - | [[Category: Pre-mrna splicing]]
| + | |
| - | [[Category: Sah]]
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| - | [[Category: Splicing]]
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| Structural highlights
Function
PR38A_HUMAN May be required for pre-mRNA splicing.
Publication Abstract from PubMed
The spliceosomal B complex-specific protein Prp38 forms a complex with the intrinsically unstructured proteins MFAP1 and Snu23. Our binding and crystal structure analyses show that MFAP1 and Snu23 contact Prp38 via ER/K motif-stabilized single alpha helices, which have previously been recognized only as rigid connectors or force springs between protein domains. A variant of the Prp38-binding single alpha helix of MFAP1, in which ER/K motifs not involved in Prp38 binding were mutated, was less alpha-helical in isolation and showed a reduced Prp38 affinity, with opposing tendencies in interaction enthalpy and entropy. Our results indicate that the strengths of single alpha helix-based interactions can be tuned by the degree of helix stabilization in the unbound state. MFAP1, Snu23, and several other spliceosomal proteins contain multiple regions that likely form single alpha helices via which they might tether several binding partners and act as intermittent scaffolds that facilitate remodeling steps during assembly of an active spliceosome.
Scaffolding in the Spliceosome via Single alpha Helices.,Ulrich AKC, Seeger M, Schutze T, Bartlick N, Wahl MC Structure. 2016 Nov 1;24(11):1972-1983. doi: 10.1016/j.str.2016.09.007. Epub 2016, Oct 20. PMID:27773687[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Ulrich AKC, Seeger M, Schutze T, Bartlick N, Wahl MC. Scaffolding in the Spliceosome via Single alpha Helices. Structure. 2016 Nov 1;24(11):1972-1983. doi: 10.1016/j.str.2016.09.007. Epub 2016, Oct 20. PMID:27773687 doi:http://dx.doi.org/10.1016/j.str.2016.09.007
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