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| | ==Crystal structure of protein Lpp20 (HP1456) from Helicobacter pylori== | | ==Crystal structure of protein Lpp20 (HP1456) from Helicobacter pylori== |
| - | <StructureSection load='5ok8' size='340' side='right' caption='[[5ok8]], [[Resolution|resolution]] 1.87Å' scene=''> | + | <StructureSection load='5ok8' size='340' side='right'caption='[[5ok8]], [[Resolution|resolution]] 1.87Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5ok8]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Campylobacter_pylori_j99 Campylobacter pylori j99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OK8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5OK8 FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5ok8]] is a 4 chain structure with sequence from [https://en.wikipedia.org/wiki/Helicobacter_pylori_J99 Helicobacter pylori J99]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5OK8 OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5OK8 FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 1.874Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">lpp20, jhp_1349 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=85963 Campylobacter pylori J99])</td></tr> | + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5ok8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ok8 OCA], [http://pdbe.org/5ok8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5ok8 RCSB], [http://www.ebi.ac.uk/pdbsum/5ok8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5ok8 ProSAT]</span></td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5ok8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5ok8 OCA], [https://pdbe.org/5ok8 PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5ok8 RCSB], [https://www.ebi.ac.uk/pdbsum/5ok8 PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5ok8 ProSAT]</span></td></tr> |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/LPP20_HELPJ LPP20_HELPJ]] Could play a role in the pathogenesis of H.pylori by serving as an inflammatory mediator. | + | [https://www.uniprot.org/uniprot/LPP20_HELPJ LPP20_HELPJ] Could play a role in the pathogenesis of H.pylori by serving as an inflammatory mediator. |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Campylobacter pylori j99]] | + | [[Category: Helicobacter pylori J99]] |
| - | [[Category: Mishra, N]] | + | [[Category: Large Structures]] |
| - | [[Category: Valesse, F]] | + | [[Category: Mishra N]] |
| - | [[Category: Zanotti, G]] | + | [[Category: Valesse F]] |
| - | [[Category: Helicobacter pylori]] | + | [[Category: Zanotti G]] |
| - | [[Category: Lipoprotein]]
| + | |
| - | [[Category: Unknown function]]
| + | |
| - | [[Category: Virulence factor]]
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| Structural highlights
Function
LPP20_HELPJ Could play a role in the pathogenesis of H.pylori by serving as an inflammatory mediator.
Publication Abstract from PubMed
BACKGROUND: Helicobacter pylori is a bacterium that affects about 50% of the world population and, despite being often asymptomatic, it is responsible of several gastric diseases, from gastritis to gastric cancer. The protein Lpp20 (HP1456) plays an important role in bacterium survival and host colonization, but the possibility that it might be involved in the etiology of H. pylori-related disorders is an unexplored issue. Lpp20 is a lipoprotein bound to the external membrane of the bacterium, but it is also secreted inside vesicles along with other two proteins of the same operon, i.e. HP1454 and HP1457. RESULTS: In this study we determined the crystal structure of Lpp20 and we found that it has a fold similar to a carcinogenic factor released by H. pylori, namely Tipalpha. We demonstrate that Lpp20 promotes cell migration and E-cadherin down-regulation in gastric cancer cells, two events recalling the epithelial-mesenchymal transition (EMT) process. Differently from Tipalpha, Lpp20 also stimulates cell proliferation. CONCLUSIONS: This identifies Lpp20 as a new pathogenic factor produced by H. pylori that promotes EMT and thereby the progression of cancer to the metastatic state.
Helicobacter pylori antigenic Lpp20 is a structural homologue of Tipalpha and promotes epithelial-mesenchymal transition.,Vallese F, Mishra NM, Pagliari M, Berto P, Codolo G, de Bernard M, Zanotti G Biochim Biophys Acta. 2017 Dec;1861(12):3263-3271. doi:, 10.1016/j.bbagen.2017.09.017. Epub 2017 Sep 22. PMID:28947343[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Vallese F, Mishra NM, Pagliari M, Berto P, Codolo G, de Bernard M, Zanotti G. Helicobacter pylori antigenic Lpp20 is a structural homologue of Tipalpha and promotes epithelial-mesenchymal transition. Biochim Biophys Acta. 2017 Dec;1861(12):3263-3271. doi:, 10.1016/j.bbagen.2017.09.017. Epub 2017 Sep 22. PMID:28947343 doi:http://dx.doi.org/10.1016/j.bbagen.2017.09.017
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