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5xsg

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Revision as of 06:58, 14 October 2020

Ultrahigh resolution structure of FUS (37-42) SYSGYS determined by MicroED

Structural highlights

5xsg is a 1 chain structure. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Disease

[FUS_HUMAN] Frontotemporal dementia with motor neuron disease;Hereditary essential tremor;Amyotrophic lateral sclerosis;Juvenile amyotrophic lateral sclerosis;Myxofibrosarcoma;Myxoid/round cell liposarcoma. A chromosomal aberration involving FUS is found in a patient with malignant myxoid liposarcoma. Translocation t(12;16)(q13;p11) with DDIT3. A chromosomal aberration involving FUS is a cause of acute myeloid leukemia (AML). Translocation t(16;21)(p11;q22) with ERG. The disease may be caused by mutations affecting the gene represented in this entry. A chromosomal aberration involving FUS is found in a patient with angiomatoid fibrous histiocytoma. Translocation t(12;16)(q13;p11.2) with ATF1 generates a chimeric FUS/ATF1 protein. The disease is caused by mutations affecting the gene represented in this entry. The disease is caused by mutations affecting the gene represented in this entry.

Function

[FUS_HUMAN] Binds both single-stranded and double-stranded DNA and promotes ATP-independent annealing of complementary single-stranded DNAs and D-loop formation in superhelical double-stranded DNA. May play a role in maintenance of genomic integrity.

Publication Abstract from PubMed

Thermostable cross-beta structures are characteristic of pathological amyloid fibrils, but these structures cannot explain the reversible nature of fibrils formed by RNA-binding proteins such as fused in sarcoma (FUS), involved in RNA granule assembly. Here, we find that two tandem (S/G)Y(S/G) motifs of the human FUS low-complexity domain (FUS LC) form reversible fibrils in a temperature- and phosphorylation-dependent manner. We named these motifs reversible amyloid cores, or RAC1 and RAC2, and determined their atomic structures in fibrillar forms, using microelectron and X-ray diffraction techniques. The RAC1 structure features an ordered-coil fibril spine rather than the extended beta-strand typical of amyloids. Ser42, a phosphorylation site of FUS, is critical in the maintenance of the ordered-coil structure, which explains how phosphorylation controls fibril formation. The RAC2 structure shows a labile fibril spine with a wet interface. These structures illuminate the mechanism of reversible fibril formation and dynamic assembly of RNA granules.

Atomic structures of FUS LC domain segments reveal bases for reversible amyloid fibril formation.,Luo F, Gui X, Zhou H, Gu J, Li Y, Liu X, Zhao M, Li D, Li X, Liu C Nat Struct Mol Biol. 2018 Apr;25(4):341-346. doi: 10.1038/s41594-018-0050-8. Epub, 2018 Apr 2. PMID:29610493^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Luo F, Gui X, Zhou H, Gu J, Li Y, Liu X, Zhao M, Li D, Li X, Liu C. Atomic structures of FUS LC domain segments reveal bases for reversible amyloid fibril formation. Nat Struct Mol Biol. 2018 Apr;25(4):341-346. doi: 10.1038/s41594-018-0050-8. Epub, 2018 Apr 2. PMID:29610493 doi:http://dx.doi.org/10.1038/s41594-018-0050-8

1 Structural highlights
2 Disease
3 Function
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/5xsg"

@@ Line 1: / Line 1: @@
 ==Ultrahigh resolution structure of FUS (37-42) SYSGYS determined by MicroED==
-<StructureSection load='5xsg' size='340' side='right' caption='[[5xsg]], [[Resolution|resolution]] 0.73&Aring;' scene=''>
+<StructureSection load='5xsg' size='340' side='right'caption='[[5xsg]], [[Resolution|resolution]] 0.73&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[5xsg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XSG OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5XSG FirstGlance]. <br>
+<table><tr><td colspan='2'>[[5xsg]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5XSG OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=5XSG FirstGlance]. <br>
-</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5xsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xsg OCA], [http://pdbe.org/5xsg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xsg RCSB], [http://www.ebi.ac.uk/pdbsum/5xsg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xsg ProSAT]</span></td></tr>
+</td></tr><tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=5xsg FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5xsg OCA], [http://pdbe.org/5xsg PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5xsg RCSB], [http://www.ebi.ac.uk/pdbsum/5xsg PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5xsg ProSAT]</span></td></tr>
 </table>
 == Disease ==
@@ Line 23: / Line 23: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Gui, X]]
 [[Category: Li, D]]

5xsg

From Proteopedia

Revision as of 06:58, 14 October 2020

Ultrahigh resolution structure of FUS (37-42) SYSGYS determined by MicroED

Structural highlights

Disease

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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