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1xgd

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Revision as of 09:58, 6 January 2021

Apo R268A human aldose reductase

Structural highlights

1xgd is a 1 chain structure with sequence from Human. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	AKR1B1, ALDR1 (HUMAN)
Activity:	Aldehyde reductase, with EC number 1.1.1.21
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[ALDR_HUMAN] Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Aldose reductase (AR) catalyzes the NADPH-dependent reduction of glucose and other sugars to their respective sugar alcohols. The NADP+/NADPH exchange is the rate-limiting step for this enzyme and contributes in varying degrees to the catalytic rates of other aldo-keto reductase superfamily enzymes. The mutation of Arg268 to alanine in human recombinant AR removes one of the ligands of the C2-phosphate of NADP+ and markedly reduces the interaction of the apoenzyme with the nucleotide. The crystal structure of human R268A apo-aldose reductase determined to a resolution of 2.1 A is described. The R268A mutant enzyme has similar kinetic parameters to the wild-type enzyme for aldehyde substrates, yet has greatly reduced affinity for the nucleotide substrate which greatly facilitates its crystallization in the apoenzyme form. The apo-structure shows that a high temperature factor loop (between residues 214 and 226) is displaced by as much as 17 A in a rigid body fashion about Gly213 and Ser226 in the absence of the nucleotide cofactor as compared to the wild-type holoenzyme structure. Several factors act to stabilize the NADPH-holding loop in either the 'open' or 'closed' conformations: (1) the presence and interactions of the nucleotide cofactor, (2) the residues surrounding the Gly213 and Ser226 hinges which form unique hydrogen bonds in the 'open' or 'closed' structure, and (3) the Trp219 "latch" residue which interacts with an arginine residue, Arg293, in the 'open' conformation or with a cysteine residue, Cys298, in the 'closed' conformation. Several mutations in and around the high temperature factor loop are examined to elucidate the role of the loop in the mechanism by which aldose reductase binds and releases its nucleotide substrate.

The structure of Apo R268A human aldose reductase: hinges and latches that control the kinetic mechanism.,Bohren KM, Brownlee JM, Milne AC, Gabbay KH, Harrison DH Biochim Biophys Acta. 2005 May 15;1748(2):201-12. PMID:15769597^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Bohren KM, Brownlee JM, Milne AC, Gabbay KH, Harrison DH. The structure of Apo R268A human aldose reductase: hinges and latches that control the kinetic mechanism. Biochim Biophys Acta. 2005 May 15;1748(2):201-12. PMID:15769597 doi:10.1016/j.bbapap.2005.01.006

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1xgd"

@@ Line 1: / Line 1: @@
 ==Apo R268A human aldose reductase==
-<StructureSection load='1xgd' size='340' side='right' caption='[[1xgd]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
+<StructureSection load='1xgd' size='340' side='right'caption='[[1xgd]], [[Resolution|resolution]] 2.10&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1xgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGD OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1XGD FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1xgd]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Human Human]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1XGD OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1XGD FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">AKR1B1, ALDR1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=9606 HUMAN])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Aldehyde_reductase Aldehyde reductase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.1.1.21 1.1.1.21] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1xgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgd OCA], [http://pdbe.org/1xgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xgd RCSB], [http://www.ebi.ac.uk/pdbsum/1xgd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgd ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1xgd FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1xgd OCA], [http://pdbe.org/1xgd PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1xgd RCSB], [http://www.ebi.ac.uk/pdbsum/1xgd PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1xgd ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 31: / Line 31: @@
 ==See Also==
-*[[Aldose Reductase|Aldose Reductase]]
+*[[Aldose reductase 3D structures|Aldose reductase 3D structures]]
 == References ==
 <references/>
@@ Line 38: / Line 38: @@
 [[Category: Aldehyde reductase]]
 [[Category: Human]]
+[[Category: Large Structures]]
 [[Category: Bohren, K M]]
 [[Category: Brownlee, J M]]

1xgd

From Proteopedia

Revision as of 09:58, 6 January 2021

Apo R268A human aldose reductase

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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