6d38

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m (Protected "6d38" [edit=sysop:move=sysop])
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'''Unreleased structure'''
 
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The entry 6d38 is ON HOLD
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==Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)==
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<StructureSection load='6d38' size='340' side='right' caption='[[6d38]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
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== Structural highlights ==
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<table><tr><td colspan='2'>[[6d38]] is a 1 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6D38 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6D38 FirstGlance]. <br>
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</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene></td></tr>
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<tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=GYC:[(4Z)-2-[(1R)-1-AMINO-2-MERCAPTOETHYL]-4-(4-HYDROXYBENZYLIDENE)-5-OXO-4,5-DIHYDRO-1H-IMIDAZOL-1-YL]ACETIC+ACID'>GYC</scene></td></tr>
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<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[6d39|6d39]]</td></tr>
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<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6d38 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6d38 OCA], [http://pdbe.org/6d38 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6d38 RCSB], [http://www.ebi.ac.uk/pdbsum/6d38 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6d38 ProSAT]</span></td></tr>
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</table>
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<div style="background-color:#fffaf0;">
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== Publication Abstract from PubMed ==
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Protein kinases transduce signals to regulate a wide array of cellular functions in eukaryotes. A generalizable method for optical control of kinases would enable fine spatiotemporal interrogation or manipulation of these various functions. We report the design and application of single-chain cofactor-free kinases with photoswitchable activity. We engineered a dimeric protein, pdDronpa, that dissociates in cyan light and reassociates in violet light. Attaching two pdDronpa domains at rationally selected locations in the kinase domain, we created the photoswitchable kinases psRaf1, psMEK1, psMEK2, and psCDK5. Using these photoswitchable kinases, we established an all-optical cell-based assay for screening inhibitors, uncovered a direct and rapid inhibitory feedback loop from ERK to MEK1, and mediated developmental changes and synaptic vesicle transport in vivo using light.
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Authors: Zhou, X., Fan, L., Lin, M.
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Optical control of cell signaling by single-chain photoswitchable kinases.,Zhou XX, Fan LZ, Li P, Shen K, Lin MZ Science. 2017 Feb 24;355(6327):836-842. doi: 10.1126/science.aah3605. PMID:28232577<ref>PMID:28232577</ref>
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Description: Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)
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From MEDLINE&reg;/PubMed&reg;, a database of the U.S. National Library of Medicine.<br>
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[[Category: Unreleased Structures]]
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</div>
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[[Category: Zhou, X]]
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<div class="pdbe-citations 6d38" style="background-color:#fffaf0;"></div>
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[[Category: Lin, M]]
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== References ==
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<references/>
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__TOC__
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</StructureSection>
[[Category: Fan, L]]
[[Category: Fan, L]]
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[[Category: Lin, M]]
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[[Category: Zhou, X]]
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[[Category: Dimer]]
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[[Category: Dronpa]]
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[[Category: Fluorescent protein]]
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[[Category: Gfp]]
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[[Category: Optogenetic]]
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[[Category: Pddronpa]]
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[[Category: Photodissociable]]

Revision as of 05:33, 16 May 2018

Photodissociable dimeric Dronpa green fluorescent protein variant M (pdDronpaM)

6d38, resolution 2.80Å

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