2het
From Proteopedia
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[[Image:2het.gif|left|200px]] | [[Image:2het.gif|left|200px]] | ||
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'''Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3''' | '''Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3''' | ||
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[[Category: Granzin, J.]] | [[Category: Granzin, J.]] | ||
[[Category: Weiergraber, O H.]] | [[Category: Weiergraber, O H.]] | ||
| - | [[Category: | + | [[Category: Calcium binding]] |
| - | [[Category: | + | [[Category: Ef-hand]] |
| - | [[Category: | + | [[Category: Helix-loop-helix]] |
| - | [[Category: | + | [[Category: Recoverin]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:12:15 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:12, 4 May 2008
Non-myristoylated bovine recoverin (truncated at C-terminus) with calcium bound to EF-hand 3
Overview
Recoverin is a Ca(2+)-regulated signal transduction modulator expressed in the vertebrate retina that has been implicated in visual adaptation. An intriguing feature of recoverin is a cluster of charged residues at its C terminus, the functional significance of which is largely unclear. To elucidate the impact of this segment on recoverin structure and function, we have investigated a mutant lacking the C-terminal 12 amino acids. Whereas in myristoylated recoverin the truncation causes an overall decrease in Ca(2+) sensitivity, results for the non-myristoylated mutant indicate that the truncation primarily affects the high affinity EF-hand 3. The three-dimensional structure of the mutant has been determined by x-ray crystallography. In addition to significant changes in average coordinates compared with wild-type recoverin, the structure provides strong indication of increased conformational flexibility, particularly in the C-terminal domain. Based on these observations, we propose a novel role of the C-terminal segment of recoverin as an internal modulator of Ca(2+) sensitivity.
About this Structure
2HET is a Single protein structure of sequence from Bos taurus. Full crystallographic information is available from OCA.
Reference
Tuning of a neuronal calcium sensor., Weiergraber OH, Senin II, Zernii EY, Churumova VA, Kovaleva NA, Nazipova AA, Permyakov SE, Permyakov EA, Philippov PP, Granzin J, Koch KW, J Biol Chem. 2006 Dec 8;281(49):37594-602. Epub 2006 Oct 2. PMID:17015448 Page seeded by OCA on Sun May 4 06:12:15 2008
