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| | ==Crystal structure of fructose-1,6-bisphosphatase T84S from Mycobacterium tuberculosis== | | ==Crystal structure of fructose-1,6-bisphosphatase T84S from Mycobacterium tuberculosis== |
| - | <StructureSection load='6ayu' size='340' side='right' caption='[[6ayu]], [[Resolution|resolution]] 2.20Å' scene=''> | + | <StructureSection load='6ayu' size='340' side='right'caption='[[6ayu]], [[Resolution|resolution]] 2.20Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[6ayu]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Mycta Mycta]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AYU OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6AYU FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[6ayu]] is a 2 chain structure with sequence from [https://en.wikipedia.org/wiki/Mycobacterium_tuberculosis_H37Ra Mycobacterium tuberculosis H37Ra]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6AYU OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=6AYU FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 2.2Å</td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">glpX, MRA_1110 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=419947 MYCTA])</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=F6P:FRUCTOSE-6-PHOSPHATE'>F6P</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=MLI:MALONATE+ION'>MLI</scene></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Fructose-bisphosphatase Fructose-bisphosphatase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.3.11 3.1.3.11] </span></td></tr>
| + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=6ayu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ayu OCA], [https://pdbe.org/6ayu PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=6ayu RCSB], [https://www.ebi.ac.uk/pdbsum/6ayu PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=6ayu ProSAT]</span></td></tr> |
| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ayu FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ayu OCA], [http://pdbe.org/6ayu PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ayu RCSB], [http://www.ebi.ac.uk/pdbsum/6ayu PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ayu ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/GLPX_MYCTA GLPX_MYCTA]] Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate. | + | [https://www.uniprot.org/uniprot/GLPX_MYCTU GLPX_MYCTU] Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate (PubMed:15470127, PubMed:21451980). Seems to be the major FBPase of M.tuberculosis and to play a key role in gluconeogenesis for conversion of lipid carbon into cell wall glycans. Does not display activity against inositol 1-phosphate (PubMed:15470127).<ref>PMID:15470127</ref> <ref>PMID:21451980</ref> |
| | <div style="background-color:#fffaf0;"> | | <div style="background-color:#fffaf0;"> |
| | == Publication Abstract from PubMed == | | == Publication Abstract from PubMed == |
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| | </div> | | </div> |
| | <div class="pdbe-citations 6ayu" style="background-color:#fffaf0;"></div> | | <div class="pdbe-citations 6ayu" style="background-color:#fffaf0;"></div> |
| | + | |
| | + | ==See Also== |
| | + | *[[Fructose-1%2C6-bisphosphatase 3D structures|Fructose-1%2C6-bisphosphatase 3D structures]] |
| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Fructose-bisphosphatase]] | + | [[Category: Large Structures]] |
| - | [[Category: Mycta]] | + | [[Category: Mycobacterium tuberculosis H37Ra]] |
| - | [[Category: Abad-Zapatero, C]] | + | [[Category: Abad-Zapatero C]] |
| - | [[Category: Gutka, H J]] | + | [[Category: Gutka HJ]] |
| - | [[Category: Movahedzadeh, F]] | + | [[Category: Movahedzadeh F]] |
| - | [[Category: Wolf, N]] | + | [[Category: Wolf N]] |
| - | [[Category: 6-bisphosphatase]]
| + | |
| - | [[Category: Class ii phosphatase]]
| + | |
| - | [[Category: Fructose-1]]
| + | |
| - | [[Category: Gluconeogenesis]]
| + | |
| - | [[Category: Hydrolase]]
| + | |
| - | [[Category: Mycobacterium tuberculosis]]
| + | |
| Structural highlights
Function
GLPX_MYCTU Catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate (PubMed:15470127, PubMed:21451980). Seems to be the major FBPase of M.tuberculosis and to play a key role in gluconeogenesis for conversion of lipid carbon into cell wall glycans. Does not display activity against inositol 1-phosphate (PubMed:15470127).[1] [2]
Publication Abstract from PubMed
The crystal structures of native class II fructose-1,6-bisphosphatase (FBPaseII) from Mycobacterium tuberculosis at 2.6 A resolution and two active-site protein variants are presented. The variants were complexed with the reaction product fructose 6-phosphate (F6P). The Thr84Ala mutant is inactive, while the Thr84Ser mutant has a lower catalytic activity. The structures reveal the presence of a 222 tetramer, similar to those described for fructose-1,6/sedoheptulose-1,7-bisphosphatase from Synechocystis (strain 6803) as well as the equivalent enzyme from Thermosynechococcus elongatus. This homotetramer corresponds to a homologous oligomer that is present but not described in the crystal structure of FBPaseII from Escherichia coli and is probably conserved in all FBPaseIIs. The constellation of amino-acid residues in the active site of FBPaseII from M. tuberculosis (MtFBPaseII) is conserved and is analogous to that described previously for the E. coli enzyme. Moreover, the structure of the active site of the partially active (Thr84Ser) variant and the analysis of the kinetics are consistent with the previously proposed catalytic mechanism. The presence of metabolites in the crystallization medium (for example citrate and malonate) and in the corresponding crystal structures of MtFBPaseII, combined with their observed inhibitory effect, could suggest the existence of an uncharacterized inhibition of this class of enzymes besides the allosteric inhibition by adenosine monophosphate observed for the Synechocystis enzyme. The structural and functional insights derived from the structure of MtFBPaseII will provide critical information for the design of lead inhibitors, which will be used to validate this target for future chemical intervention.
Structures of the Mycobacterium tuberculosis GlpX protein (class II fructose-1,6-bisphosphatase): implications for the active oligomeric state, catalytic mechanism and citrate inhibition.,Wolf NM, Gutka HJ, Movahedzadeh F, Abad-Zapatero C Acta Crystallogr D Struct Biol. 2018 Apr 1;74(Pt 4):321-331. doi:, 10.1107/S2059798318002838. Epub 2018 Apr 3. PMID:29652259[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Movahedzadeh F, Rison SC, Wheeler PR, Kendall SL, Larson TJ, Stoker NG. The Mycobacterium tuberculosis Rv1099c gene encodes a GlpX-like class II fructose 1,6-bisphosphatase. Microbiology (Reading). 2004 Oct;150(Pt 10):3499-505. PMID:15470127 doi:10.1099/mic.0.27204-0
- ↑ Gutka HJ, Rukseree K, Wheeler PR, Franzblau SG, Movahedzadeh F. glpX gene of Mycobacterium tuberculosis: heterologous expression, purification, and enzymatic characterization of the encoded fructose 1,6-bisphosphatase II. Appl Biochem Biotechnol. 2011 Aug;164(8):1376-89. PMID:21451980 doi:10.1007/s12010-011-9219-x
- ↑ Wolf NM, Gutka HJ, Movahedzadeh F, Abad-Zapatero C. Structures of the Mycobacterium tuberculosis GlpX protein (class II fructose-1,6-bisphosphatase): implications for the active oligomeric state, catalytic mechanism and citrate inhibition. Acta Crystallogr D Struct Biol. 2018 Apr 1;74(Pt 4):321-331. doi:, 10.1107/S2059798318002838. Epub 2018 Apr 3. PMID:29652259 doi:http://dx.doi.org/10.1107/S2059798318002838
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