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1z19

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Revision as of 08:56, 20 January 2021

Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site

Structural highlights

1z19 is a 5 chain structure with sequence from Bacteriophage lambda. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
NonStd Res:	,
Related:	1p7d
Gene:	INT (Bacteriophage lambda)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[VINT_LAMBD] Integrase is necessary for integration of the phage into the host genome by site-specific recombination. In conjunction with excisionase, integrase is also necessary for excision of the prophage from the host genome.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Site-specific DNA recombination is important for basic cellular functions including viral integration, control of gene expression, production of genetic diversity and segregation of newly replicated chromosomes, and is used by bacteriophage lambda to integrate or excise its genome into and out of the host chromosome. lambda recombination is carried out by the bacteriophage-encoded integrase protein (lambda-int) together with accessory DNA sites and associated bending proteins that allow regulation in response to cell physiology. Here we report the crystal structures of lambda-int in higher-order complexes with substrates and regulatory DNAs representing different intermediates along the reaction pathway. The structures show how the simultaneous binding of two separate domains of lambda-int to DNA facilitates synapsis and can specify the order of DNA strand cleavage and exchange. An intertwined layer of amino-terminal domains bound to accessory (arm) DNAs shapes the recombination complex in a way that suggests how arm binding shifts the reaction equilibrium in favour of recombinant products.

A structural basis for allosteric control of DNA recombination by lambda integrase.,Biswas T, Aihara H, Radman-Livaja M, Filman D, Landy A, Ellenberger T Nature. 2005 Jun 23;435(7045):1059-66. PMID:15973401^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Biswas T, Aihara H, Radman-Livaja M, Filman D, Landy A, Ellenberger T. A structural basis for allosteric control of DNA recombination by lambda integrase. Nature. 2005 Jun 23;435(7045):1059-66. PMID:15973401 doi:http://dx.doi.org/10.1038/nature03657

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1z19"

@@ Line 1: / Line 1: @@
 ==Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site==
-<StructureSection load='1z19' size='340' side='right' caption='[[1z19]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
+<StructureSection load='1z19' size='340' side='right'caption='[[1z19]], [[Resolution|resolution]] 2.80&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1z19]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z19 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1Z19 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1z19]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacteriophage_lambda Bacteriophage lambda]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1Z19 OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1Z19 FirstGlance]. <br>
 </td></tr><tr id='NonStdRes'><td class="sblockLbl"><b>[[Non-Standard_Residue|NonStd Res:]]</b></td><td class="sblockDat"><scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>, <scene name='pdbligand=PTR:O-PHOSPHOTYROSINE'>PTR</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1p7d|1p7d]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1p7d|1p7d]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">INT ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10710 Bacteriophage lambda])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1z19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z19 OCA], [http://pdbe.org/1z19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z19 RCSB], [http://www.ebi.ac.uk/pdbsum/1z19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z19 ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1z19 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1z19 OCA], [http://pdbe.org/1z19 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1z19 RCSB], [http://www.ebi.ac.uk/pdbsum/1z19 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1z19 ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 32: / Line 32: @@
 ==See Also==
-*[[Phage integrase|Phage integrase]]
+*[[Retroviral integrase 3D structures|Retroviral integrase 3D structures]]
-*[[Resolvase|Resolvase]]
 == References ==
 <references/>
@@ Line 39: / Line 38: @@
 </StructureSection>
 [[Category: Bacteriophage lambda]]
+[[Category: Large Structures]]
 [[Category: Aihara, H]]
 [[Category: Biswas, T]]

1z19

From Proteopedia

Revision as of 08:56, 20 January 2021

Crystal structure of a lambda integrase(75-356) dimer bound to a COC' core site

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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