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1yyk

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Revision as of 08:55, 20 January 2021

Crystal structure of RNase III from Aquifex Aeolicus complexed with double-stranded RNA at 2.5-angstrom resolution

Structural highlights

1yyk is a 6 chain structure with sequence from "aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	1i4s, 1jfz, 1o0w, 1rc5, 1rc7, 1yyo, 1yyw, 1yz9
Gene:	rnc ("Aquifex aeolicus" Huber and Stetter 2001)
Activity:	Ribonuclease III, with EC number 3.1.26.3
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[RNC_AQUAE] Digests double-stranded RNA. Involved in the processing of primary rRNA transcript to yield the immediate precursors to the large and small rRNAs (23S and 16S). Also processes some mRNAs, and tRNAs when they are encoded in the rRNA operon.^[1] ^[2]

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Bacterial ribonuclease III (RNase III) can affect RNA structure and gene expression in either of two ways: as a processing enzyme that cleaves double-stranded (ds) RNA, or as a binding protein that binds but does not cleave dsRNA. We previously proposed a model of the catalytic complex of RNase III with dsRNA based on three crystal structures, including the endonuclease domain of RNase III with and without bound metal ions and a dsRNA binding protein complexed with dsRNA. We also reported a noncatalytic assembly observed in the crystal structure of an RNase III mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We hypothesize that the RNase III*dsRNA complex can exist in two functional forms, a catalytic complex and a noncatalytic assembly, and that in between the two forms there may be intermediate states. Here, we present four crystal structures of RNase III complexed with dsRNA, representing possible intermediates.

Intermediate states of ribonuclease III in complex with double-stranded RNA.,Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X Structure. 2005 Oct;13(10):1435-42. PMID:16216575^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Shi Z, Nicholson RH, Jaggi R, Nicholson AW. Characterization of Aquifex aeolicus ribonuclease III and the reactivity epitopes of its pre-ribosomal RNA substrates. Nucleic Acids Res. 2011 Apr;39(7):2756-68. doi: 10.1093/nar/gkq1030. Epub 2010, Dec 7. PMID:21138964 doi:http://dx.doi.org/10.1093/nar/gkq1030
↑ Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X. Structural insight into the mechanism of double-stranded RNA processing by ribonuclease III. Cell. 2006 Jan 27;124(2):355-66. PMID:16439209 doi:10.1016/j.cell.2005.11.034
↑ Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X. Intermediate states of ribonuclease III in complex with double-stranded RNA. Structure. 2005 Oct;13(10):1435-42. PMID:16216575 doi:10.1016/j.str.2005.06.014

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1yyk"

@@ Line 1: / Line 1: @@
 ==Crystal structure of RNase III from Aquifex Aeolicus complexed with double-stranded RNA at 2.5-angstrom resolution==
-<StructureSection load='1yyk' size='340' side='right' caption='[[1yyk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
+<StructureSection load='1yyk' size='340' side='right'caption='[[1yyk]], [[Resolution|resolution]] 2.50&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1yyk]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YYK FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1yyk]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"aquifex_aeolicus"_huber_and_stetter_2001 "aquifex aeolicus" huber and stetter 2001]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YYK OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YYK FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene></td></tr>
-<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[1i4s|1i4s]], [[1jfz|1jfz]], [[1o0w|1o0w]], [[1rc5|1rc5]], [[1rc7|1rc7]], [[1yyo|1yyo]], [[1yyw|1yyw]], [[1yz9|1yz9]]</td></tr>
+<tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat"><div style='overflow: auto; max-height: 3em;'>[[1i4s|1i4s]], [[1jfz|1jfz]], [[1o0w|1o0w]], [[1rc5|1rc5]], [[1rc7|1rc7]], [[1yyo|1yyo]], [[1yyw|1yyw]], [[1yz9|1yz9]]</div></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">rnc ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=63363 "Aquifex aeolicus" Huber and Stetter 2001])</td></tr>
 <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Ribonuclease_III Ribonuclease III], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.26.3 3.1.26.3] </span></td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1yyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yyk OCA], [http://pdbe.org/1yyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yyk RCSB], [http://www.ebi.ac.uk/pdbsum/1yyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yyk ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1yyk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1yyk OCA], [http://pdbe.org/1yyk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1yyk RCSB], [http://www.ebi.ac.uk/pdbsum/1yyk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1yyk ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 ==See Also==
-*[[Ribonuclease|Ribonuclease]]
+*[[Ribonuclease 3D structures|Ribonuclease 3D structures]]
 *[[Temp|Temp]]
 == References ==
@@ Line 40: / Line 40: @@
 </StructureSection>
 [[Category: Aquifex aeolicus huber and stetter 2001]]
+[[Category: Large Structures]]
 [[Category: Ribonuclease III]]
 [[Category: Austin, B P]]

1yyk

From Proteopedia

Revision as of 08:55, 20 January 2021

Crystal structure of RNase III from Aquifex Aeolicus complexed with double-stranded RNA at 2.5-angstrom resolution

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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