2hii

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2hii.jpg|left|200px]]
[[Image:2hii.jpg|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2hii |SIZE=350|CAPTION= <scene name='initialview01'>2hii</scene>, resolution 2.79&Aring;
+
The line below this paragraph, containing "STRUCTURE_2hii", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=MSE:SELENOMETHIONINE'>MSE</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= pcnB, pcnA-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]), pcnC, pcnA-2 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus]), pcnA, pcnA-1 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2287 Sulfolobus solfataricus])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2hii| PDB=2hii | SCENE= }}
-
|RELATEDENTRY=[[2hik|2HIK]], [[2hiv|2HIV]], [[2hix|2HIX]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hii FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hii OCA], [http://www.ebi.ac.uk/pdbsum/2hii PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hii RCSB]</span>
+
-
}}
+
'''heterotrimeric PCNA sliding clamp'''
'''heterotrimeric PCNA sliding clamp'''
Line 28: Line 25:
[[Category: Pascal, J M.]]
[[Category: Pascal, J M.]]
[[Category: Tsodikov, O V.]]
[[Category: Tsodikov, O V.]]
-
[[Category: dna replication]]
+
[[Category: Dna replication]]
-
[[Category: heterotrimeric]]
+
[[Category: Heterotrimeric]]
-
[[Category: processivity factor]]
+
[[Category: Processivity factor]]
-
[[Category: sliding clamp]]
+
[[Category: Sliding clamp]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:20:11 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:30:55 2008''
+

Revision as of 03:20, 4 May 2008

Image:2hii.jpg

Template:STRUCTURE 2hii

heterotrimeric PCNA sliding clamp


Overview

DNA sliding clamps encircle DNA and provide binding sites for many DNA-processing enzymes. However, it is largely unknown how sliding clamps like proliferating cell nuclear antigen (PCNA) coordinate multistep DNA transactions. We have determined structures of Sulfolobus solfataricus DNA ligase and heterotrimeric PCNA separately by X-ray diffraction and in complex by small-angle X-ray scattering (SAXS). Three distinct PCNA subunits assemble into a protein ring resembling the homotrimeric PCNA of humans but with three unique protein-binding sites. In the absence of nicked DNA, the Sulfolobus solfataricus DNA ligase has an open, extended conformation. When complexed with heterotrimeric PCNA, the DNA ligase binds to the PCNA3 subunit and ligase retains an open, extended conformation. A closed, ring-shaped conformation of ligase catalyzes a DNA end-joining reaction that is strongly stimulated by PCNA. This open-to-closed switch in the conformation of DNA ligase is accommodated by a malleable interface with PCNA that serves as an efficient platform for DNA ligation.

About this Structure

2HII is a Protein complex structure of sequences from Sulfolobus solfataricus. Full crystallographic information is available from OCA.

Reference

A flexible interface between DNA ligase and PCNA supports conformational switching and efficient ligation of DNA., Pascal JM, Tsodikov OV, Hura GL, Song W, Cotner EA, Classen S, Tomkinson AE, Tainer JA, Ellenberger T, Mol Cell. 2006 Oct 20;24(2):279-91. PMID:17052461 Page seeded by OCA on Sun May 4 06:20:11 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2hii"
Personal tools