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1ywm

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Revision as of 08:54, 20 January 2021

Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein

Structural highlights

1ywm is a 1 chain structure with sequence from Stra1. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	,
Gene:	bca (STRA1)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[BCA_STRA1] May play a role in both virulence and immunity.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Group B Streptococcus (GBS) is the leading cause of bacterial pneumonia, sepsis, and meningitis among neonates and an important cause of morbidity among pregnant women and immunocompromised adults. Invasive diseases due to GBS are attributed to the ability of the pathogen to translocate across human epithelial surfaces. The alpha C protein (ACP) has been identified as an invasin that plays a role in internalization and translocation of GBS across epithelial cells. The soluble N-terminal domain of ACP (NtACP) blocks the internalization of GBS. We determined the 1.86-A resolution crystal structure of NtACP comprising residues Ser(52) through Leu(225) of the full-length ACP. NtACP has two domains, an N-terminal beta-sandwich and a C-terminal three-helix bundle. Structural and topological alignments reveal that the beta-sandwich shares structural elements with the type III fibronectin fold (FnIII), but includes structural elaborations that make it unique. We have identified a potential integrin-binding motif consisting of Lys-Thr-Asp(146), Arg(110), and Asp(118). A similar arrangement of charged residues has been described in other invasins. ACP shows a heparin binding activity that requires NtACP. We propose a possible heparin-binding site, including one surface of the three-helix bundle, and nearby portions of the sandwich and repeat domains. We have validated this prediction using assays of the heparin binding and cell-adhesion properties of engineered fragments of ACP. This is the first crystal structure of a member of the highly conserved Gram-positive surface alpha-like protein family, and it will enable the internalization mechanism of GBS to be dissected at the atomic level.

Crystal structure of the N-terminal domain of the group B streptococcus alpha C protein.,Auperin TC, Bolduc GR, Baron MJ, Heroux A, Filman DJ, Madoff LC, Hogle JM J Biol Chem. 2005 May 6;280(18):18245-52. Epub 2005 Mar 6. PMID:15753100^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Auperin TC, Bolduc GR, Baron MJ, Heroux A, Filman DJ, Madoff LC, Hogle JM. Crystal structure of the N-terminal domain of the group B streptococcus alpha C protein. J Biol Chem. 2005 May 6;280(18):18245-52. Epub 2005 Mar 6. PMID:15753100 doi:10.1074/jbc.M412391200

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ywm"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein==
-<StructureSection load='1ywm' size='340' side='right' caption='[[1ywm]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
+<StructureSection load='1ywm' size='340' side='right'caption='[[1ywm]], [[Resolution|resolution]] 1.86&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ywm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stra1 Stra1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YWM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ywm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Stra1 Stra1]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YWM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YWM FirstGlance]. <br>
-</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+</td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=DTU:(2R,3S)-1,4-DIMERCAPTOBUTANE-2,3-DIOL'>DTU</scene>, <scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
 <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">bca ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=205921 STRA1])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ywm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ywm OCA], [http://pdbe.org/1ywm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ywm RCSB], [http://www.ebi.ac.uk/pdbsum/1ywm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ywm ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ywm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ywm OCA], [http://pdbe.org/1ywm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ywm RCSB], [http://www.ebi.ac.uk/pdbsum/1ywm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ywm ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 33: / Line 33: @@
 __TOC__
 </StructureSection>
+[[Category: Large Structures]]
 [[Category: Stra1]]
 [[Category: Auperin, T C]]

1ywm

From Proteopedia

Revision as of 08:54, 20 January 2021

Crystal structure of the N-terminal domain of group B Streptococcus alpha C protein

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

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