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1ygm

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Revision as of 08:45, 20 January 2021

NMR structure of Mistic

Structural highlights

1ygm is a 1 chain structure with sequence from Bacsu. Full experimental information is available from OCA. For a guided tour on the structure components use FirstGlance.
Gene:	YugO-a (BACSU)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[MSTX_BACSU] Chaperone that facilitates the production and integration of integral membrane proteins into the bacterial lipid bilayer.^[1]

Publication Abstract from PubMed

Although structure determination of soluble proteins has become routine, our understanding of membrane proteins has been limited by experimental bottlenecks in obtaining both sufficient yields of protein and ordered crystals. Mistic is an unusual Bacillus subtilis integral membrane protein that folds autonomously into the membrane, bypassing the cellular translocon machinery. Using paramagnetic probes, we determined by nuclear magnetic resonance (NMR) spectroscopy that the protein forms a helical bundle with a surprisingly polar lipid-facing surface. Additional experiments suggest that Mistic can be used for high-level production of other membrane proteins in their native conformations, including many eukaryotic proteins that have previously been intractable to bacterial expression.

NMR structure of Mistic, a membrane-integrating protein for membrane protein expression.,Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S. NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457 doi:http://dx.doi.org/307/5713/1317
↑ Roosild TP, Greenwald J, Vega M, Castronovo S, Riek R, Choe S. NMR structure of Mistic, a membrane-integrating protein for membrane protein expression. Science. 2005 Feb 25;307(5713):1317-21. PMID:15731457 doi:http://dx.doi.org/307/5713/1317

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/1ygm"

@@ Line 1: / Line 1: @@
 ==NMR structure of Mistic==
-<StructureSection load='1ygm' size='340' side='right' caption='[[1ygm]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
+<StructureSection load='1ygm' size='340' side='right'caption='[[1ygm]], [[NMR_Ensembles_of_Models | 10 NMR models]]' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
+<table><tr><td colspan='2'>[[1ygm]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacsu Bacsu]. Full experimental information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YGM OCA]. For a <b>guided tour on the structure components</b> use [http://proteopedia.org/fgij/fg.htm?mol=1YGM FirstGlance]. <br>
 </td></tr><tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">YugO-a ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=224308 BACSU])</td></tr>
-<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
+<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://proteopedia.org/fgij/fg.htm?mol=1ygm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=1ygm OCA], [http://pdbe.org/1ygm PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=1ygm RCSB], [http://www.ebi.ac.uk/pdbsum/1ygm PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=1ygm ProSAT]</span></td></tr>
 </table>
 == Function ==
@@ Line 23: / Line 23: @@
 </StructureSection>
 [[Category: Bacsu]]
+[[Category: Large Structures]]
 [[Category: Castronovo, S]]
 [[Category: Choe, S]]

1ygm

From Proteopedia

Revision as of 08:45, 20 January 2021

NMR structure of Mistic

Structural highlights

Function

Publication Abstract from PubMed

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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