1w4t
From Proteopedia
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| - | [[Image:1w4t.gif|left|200px]]<br /> | + | [[Image:1w4t.gif|left|200px]]<br /><applet load="1w4t" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1w4t" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1w4t, resolution 1.950Å" /> | caption="1w4t, resolution 1.950Å" /> | ||
'''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF PSEUDOMONAS AERUGINOSA ARYLAMINE N-ACETYLTRANSFERASE'''<br /> | '''X-RAY CRYSTALLOGRAPHIC STRUCTURE OF PSEUDOMONAS AERUGINOSA ARYLAMINE N-ACETYLTRANSFERASE'''<br /> | ||
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==About this Structure== | ==About this Structure== | ||
| - | 1W4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. | + | 1W4T is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/ ] with SO4 as [http://en.wikipedia.org/wiki/ligand ligand]. Known structural/functional Site: <scene name='pdbsite=AC1:So4 Binding Site For Chain A'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W4T OCA]. |
==Reference== | ==Reference== | ||
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[[Category: xenobiotic metabolism]] | [[Category: xenobiotic metabolism]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:30:21 2007'' |
Revision as of 16:20, 18 December 2007
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X-RAY CRYSTALLOGRAPHIC STRUCTURE OF PSEUDOMONAS AERUGINOSA ARYLAMINE N-ACETYLTRANSFERASE
Overview
The gene for NAT (arylamine N-acetyltransferase) from Pseudomonas, aeruginosa (panat) has been cloned from genomic DNA, and the gene product, (PANAT) expressed as an N-terminal histidine-tagged protein in Escherichia, coli and purified via nickel ion affinity chromatography. The specific, activities of PANAT against a broad range of substrates have been, investigated and compared with those of other prokaryotic NAT enzymes. For, most arylamine substrates identified, PANAT exhibits in vitro specific, activities typically one order of magnitude greater than those of, recombinant NAT enzymes from Mycobacterium smegmatis or Salmonella, typhimurium. Among the substrates of PANAT so far identified are the, anti-tubercular drug isoniazid, 5-aminosalicylate (a drug used in the, treatment of inflammatory bowel disease), as well as important, environmental pollutants such as 3,4-dichloroaniline and 2-aminofluorene., As well as acetylating common NAT substrates, PANAT is unique among the, prokaryotic NATs so far studied in acetylating the folate precursor, 4-aminobenzoic acid and the folate catabolite 4-aminobenzoylglutamate. The, recombinant protein has been expressed in sufficient quantity to allow, protein crystallization, and we have subsequently determined the 1.95 A, structure of PANAT by X-ray crystallography.
About this Structure
1W4T is a Single protein structure of sequence from [1] with SO4 as ligand. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Expression, purification, characterization and structure of Pseudomonas aeruginosa arylamine N-acetyltransferase., Westwood IM, Holton SJ, Rodrigues-Lima F, Dupret JM, Bhakta S, Noble ME, Sim E, Biochem J. 2005 Jan 15;385(Pt 2):605-12. PMID:15447630
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