2hji

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[[Image:2hji.gif|left|200px]]
[[Image:2hji.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2hji |SIZE=350|CAPTION= <scene name='initialview01'>2hji</scene>
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The line below this paragraph, containing "STRUCTURE_2hji", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=FE2:FE+(II)+ION'>FE2</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Acireductone_dioxygenase_(Fe(2+)-requiring) Acireductone dioxygenase (Fe(2+)-requiring)], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=1.13.11.54 1.13.11.54] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2hji| PDB=2hji | SCENE= }}
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|RELATEDENTRY=[[1zrr|1ZRR]], [[1vr3|1VR3]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2hji FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2hji OCA], [http://www.ebi.ac.uk/pdbsum/2hji PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2hji RCSB]</span>
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}}
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'''Structural model for the Fe-containing isoform of acireductone dioxygenase'''
'''Structural model for the Fe-containing isoform of acireductone dioxygenase'''
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==Reference==
==Reference==
One protein, two enzymes revisited: a structural entropy switch interconverts the two isoforms of acireductone dioxygenase., Ju T, Goldsmith RB, Chai SC, Maroney MJ, Pochapsky SS, Pochapsky TC, J Mol Biol. 2006 Nov 3;363(4):823-34. Epub 2006 Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16989860 16989860]
One protein, two enzymes revisited: a structural entropy switch interconverts the two isoforms of acireductone dioxygenase., Ju T, Goldsmith RB, Chai SC, Maroney MJ, Pochapsky SS, Pochapsky TC, J Mol Biol. 2006 Nov 3;363(4):823-34. Epub 2006 Aug 26. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/16989860 16989860]
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[[Category: Acireductone dioxygenase (Fe(2+)-requiring)]]
 
[[Category: Klebsiella oxytoca]]
[[Category: Klebsiella oxytoca]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Maroney, M J.]]
[[Category: Maroney, M J.]]
[[Category: Pochapsky, T C.]]
[[Category: Pochapsky, T C.]]
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[[Category: dioxygenase]]
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[[Category: Dioxygenase]]
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[[Category: isozyme]]
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[[Category: Isozyme]]
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[[Category: methionine salvage]]
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[[Category: Methionine salvage]]
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[[Category: non-heme iron]]
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[[Category: Non-heme iron]]
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[[Category: structural entropy]]
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[[Category: Structural entropy]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:22:02 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:31:15 2008''
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Revision as of 03:22, 4 May 2008

Template:STRUCTURE 2hji

Structural model for the Fe-containing isoform of acireductone dioxygenase


Overview

Acireductone dioxygenase (ARD) catalyzes different reactions between O2 and 1,2-dihydroxy-3-oxo-5-(methylthio)pent-1-ene (acireductone) depending upon the metal bound in the active site. Ni2+ -ARD cleaves acireductone to formate, CO and methylthiopropionate. If Fe2+ is bound (ARD'), the same substrates yield methylthioketobutyrate and formate. The two forms differ in structure, and are chromatographically separable. Paramagnetism of Fe2+ renders the active site of ARD' inaccessible to standard NMR methods. The structure of ARD' has been determined using Fe2+ binding parameters determined by X-ray absorption spectroscopy and NMR restraints from H98S ARD, a metal-free diamagnetic protein that is isostructural with ARD'. ARD' retains the beta-sandwich fold of ARD, but a structural entropy switch increases order at one end of a two-helix system that bisects the beta-sandwich and decreases order at the other upon interconversion of ARD and ARD', causing loss of the C-terminal helix in ARD' and rearrangements of residues involved in substrate orientation in the active site.

About this Structure

2HJI is a Single protein structure of sequence from Klebsiella oxytoca. Full crystallographic information is available from OCA.

Reference

One protein, two enzymes revisited: a structural entropy switch interconverts the two isoforms of acireductone dioxygenase., Ju T, Goldsmith RB, Chai SC, Maroney MJ, Pochapsky SS, Pochapsky TC, J Mol Biol. 2006 Nov 3;363(4):823-34. Epub 2006 Aug 26. PMID:16989860 Page seeded by OCA on Sun May 4 06:22:02 2008

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