This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
User:Khadar Abdi/Sandbox1
From Proteopedia
(Difference between revisions)
| Line 3: | Line 3: | ||
== Function/Mechanism == | == Function/Mechanism == | ||
| - | '''Threonyl t-RNA Synthetase''' or '''Threonyl-tRNA ligase''' or '''TARS''' is class II '''Aminoacyl-tRNA synthetase''' enzymes. These enzymes primary function are The main function of the enzyme is to add Threonine amino acid (Thr) to threonine specific tRNA (tRNA-thr) a necessity prep for the protein synthesis pathway. Below displays the overview of the Aminoacylation rxn<ref>PMID:29305884</ref> | + | '''Threonyl t-RNA Synthetase''' or '''Threonyl-tRNA ligase''' or '''TARS''' is class II '''Aminoacyl-tRNA synthetase''' enzymes. These enzymes primary function are The main function of the enzyme is to add Threonine amino acid (Thr) to threonine specific tRNA (tRNA-thr) a necessity prep for the protein synthesis pathway. Below displays the overview of the Aminoacylation rxn<ref>PMID:29305884</ref>[[Image:TARS_protein_rxn.jpeg |left|thumb|500px| '''Overall TARS protein rxn. Substrates includes Adenosine triphosphate (ATP), Threonine (Thr) and threonine specific transfer Ribonucleic Acid (tRNA-thr).''']] |
| - | + | ||
| - | [[Image:TARS_protein_rxn.jpeg |left|thumb|500px| '''Overall TARS protein rxn. Substrates includes Adenosine triphosphate (ATP), Threonine (Thr) and threonine specific transfer Ribonucleic Acid (tRNA-thr).''']] | + | |
{{Clear}} | {{Clear}} | ||
| - | TARS adds amino acid to tRNA by a two-step mechanism. First the enzyme binds to both Threonine and ATP in the catalytic domain to perform an adenylation reaction in which pyrophosphate is released as a byproduct. This is then follow up by a transferring Thr from Adenosine monophosphate molecule to 3'OH site of tRNA-thr. <ref>Lehninger, A. L., Nelson, D. L., & Cox, M. M. (2000). ''Lehninger principles of biochemistry.'' New York: Worth Publishers.</ref> Image below demonstrates the arrow pushing occuring to generate threonine bound tRNA-thr.[[Image:TARSmechanism.jpg]] | + | TARS adds amino acid to tRNA by a two-step mechanism. First the enzyme binds to both Threonine and ATP in the catalytic domain to perform an adenylation reaction in which pyrophosphate is released as a byproduct. This is then follow up by a transferring Thr from Adenosine monophosphate molecule to 3'OH site of tRNA-thr. <ref>Lehninger, A. L., Nelson, D. L., & Cox, M. M. (2000). ''Lehninger principles of biochemistry.'' New York: Worth Publishers.</ref> Image below demonstrates the arrow pushing occuring to generate threonine bound tRNA-thr. |
| + | |||
| + | [[Image:TARSmechanism.jpg]] | ||
Revision as of 18:37, 28 April 2018
Threonyl-tRNA Synthetase/ligase
| |||||||||||
