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== Function/Mechanism == | == Function/Mechanism == | ||
| - | '''Threonyl t-RNA Synthetase''' or '''Threonyl-tRNA ligase''' or '''TARS''' is class II '''Aminoacyl-tRNA synthetase''' enzymes. These enzymes primary function are The main function of the enzyme is to add Threonine amino acid (Thr) to threonine specific tRNA (tRNA-thr) a necessity prep for the protein synthesis pathway. Below displays the overview of the Aminoacylation rxn<ref>PMID:29305884</ref>[[Image:TARS_protein_rxn.jpeg |left|thumb|500px| '''Overall TARS protein rxn. Substrates includes Adenosine triphosphate (ATP), Threonine (Thr) and threonine specific transfer Ribonucleic Acid (tRNA-thr).''']] | + | '''Threonyl t-RNA Synthetase''' or '''Threonyl-tRNA ligase''' or '''TARS''' is class II '''Aminoacyl-tRNA synthetase''' enzymes. These enzymes primary function are to added the respective amino acid to the respective transfer Ribonucleic Acid (tRNA-AA) The main function of the enzyme is to add Threonine amino acid (Thr) to threonine specific tRNA (tRNA-thr) a necessity prep for the protein synthesis pathway. Below displays the overview of the Aminoacylation rxn <ref>PMID:29305884</ref>[[Image:TARS_protein_rxn.jpeg |left|thumb|500px| '''Overall TARS protein rxn. Substrates includes Adenosine triphosphate (ATP), Threonine (Thr) and threonine specific transfer Ribonucleic Acid (tRNA-thr).''']] |
{{Clear}} | {{Clear}} | ||
| - | TARS adds amino acid to tRNA by a two-step mechanism. First the enzyme binds to both Threonine and | + | TARS adds amino acid to tRNA by a two-step mechanism. First the enzyme binds to both <scene name='Triose_Phosphate_Isomerase/Morph/1'> Threonine </scene> and in the catalytic domain to perform an adenylation reaction in which pyrophosphate is released as a byproduct. This is then follow up by a transferring Thr from Adenosine monophosphate molecule to 3'OH site of tRNA-thr. <ref>Lehninger, A. L., Nelson, D. L., & Cox, M. M. (2000). ''Lehninger principles of biochemistry.'' New York: Worth Publishers.</ref> Image below demonstrates the arrow pushing occuring to generate threonine bound tRNA-thr. |
| - | [[Image:TARSmechanism.jpg]] | + | [[Image:TARSmechanism.jpg|left|thumb|300px|'''Arrow pushing of Aminoacylation rxn.<ref>Lehninger, A. L., Nelson, D. L., & Cox, M. M. (2000). ''Lehninger principles of biochemistry.'' New York: Worth Publishers.</ref> ''']] |
Revision as of 19:03, 28 April 2018
Threonyl-tRNA Synthetase/ligase
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