1w58
From Proteopedia
| Line 1: | Line 1: | ||
| - | [[Image:1w58.gif|left|200px]]<br /> | + | [[Image:1w58.gif|left|200px]]<br /><applet load="1w58" size="450" color="white" frame="true" align="right" spinBox="true" |
| - | <applet load="1w58" size="450" color="white" frame="true" align="right" spinBox="true" | + | |
caption="1w58, resolution 2.5Å" /> | caption="1w58, resolution 2.5Å" /> | ||
'''FTSZ GMPCPP SOAK I213 (M. JANNASCHII)'''<br /> | '''FTSZ GMPCPP SOAK I213 (M. JANNASCHII)'''<br /> | ||
| Line 8: | Line 7: | ||
==About this Structure== | ==About this Structure== | ||
| - | 1W58 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MG and G2P as [http://en.wikipedia.org/wiki/ligands ligands]. | + | 1W58 is a [http://en.wikipedia.org/wiki/Single_protein Single protein] structure of sequence from [http://en.wikipedia.org/wiki/Methanocaldococcus_jannaschii Methanocaldococcus jannaschii] with MG and G2P as [http://en.wikipedia.org/wiki/ligands ligands]. Known structural/functional Site: <scene name='pdbsite=AC1:Mg Binding Site For Chain 1'>AC1</scene>. Full crystallographic information is available from [http://ispc.weizmann.ac.il/oca-bin/ocashort?id=1W58 OCA]. |
==Reference== | ==Reference== | ||
| Line 29: | Line 28: | ||
[[Category: tubulin]] | [[Category: tubulin]] | ||
| - | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on | + | ''Page seeded by [http://ispc.weizmann.ac.il/oca OCA ] on Tue Dec 18 18:30:55 2007'' |
Revision as of 16:21, 18 December 2007
|
FTSZ GMPCPP SOAK I213 (M. JANNASCHII)
Overview
The prokaryotic tubulin homolog FtsZ polymerizes into a ring structure, essential for bacterial cell division. We have used refolded FtsZ to, crystallize a tubulin-like protofilament. The N- and C-terminal domains of, two consecutive subunits in the filament assemble to form the GTPase site, with the C-terminal domain providing water-polarizing residues. A, domain-swapped structure of FtsZ and biochemical data on purified N- and, C-terminal domains show that they are independent. This leads to a model, of how FtsZ and tubulin polymerization evolved by fusing two domains. In, polymerized tubulin, the nucleotide-binding pocket is occluded, which, leads to nucleotide exchange being the rate-limiting step and to dynamic, instability. In our FtsZ filament structure the nucleotide is, exchangeable, explaining why, in this filament, nucleotide hydrolysis is, the rate-limiting step during FtsZ polymerization. Furthermore, crystal, structures of FtsZ in different nucleotide states reveal notably few, differences.
About this Structure
1W58 is a Single protein structure of sequence from Methanocaldococcus jannaschii with MG and G2P as ligands. Known structural/functional Site: . Full crystallographic information is available from OCA.
Reference
Structural insights into FtsZ protofilament formation., Oliva MA, Cordell SC, Lowe J, Nat Struct Mol Biol. 2004 Dec;11(12):1243-50. Epub 2004 Nov 21. PMID:15558053
Page seeded by OCA on Tue Dec 18 18:30:55 2007
