2hu9
From Proteopedia
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[[Image:2hu9.jpg|left|200px]] | [[Image:2hu9.jpg|left|200px]] | ||
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'''X-ray structure of the Archaeoglobus fulgidus CopZ N-terminal Domain''' | '''X-ray structure of the Archaeoglobus fulgidus CopZ N-terminal Domain''' | ||
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[[Category: Rosenzweig, A C.]] | [[Category: Rosenzweig, A C.]] | ||
[[Category: Sazinsky, M H.]] | [[Category: Sazinsky, M H.]] | ||
| - | [[Category: | + | [[Category: Atox1]] |
| - | [[Category: | + | [[Category: Atx1]] |
| - | [[Category: | + | [[Category: Copper chaperone]] |
| - | [[Category: | + | [[Category: Copz]] |
| - | [[Category: | + | [[Category: Iron-sufur protein]] |
| - | [[Category: | + | [[Category: Metal transport]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:43:02 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:43, 4 May 2008
X-ray structure of the Archaeoglobus fulgidus CopZ N-terminal Domain
Overview
Bacterial CopZ proteins deliver copper to P1B-type Cu+-ATPases that are homologous to the human Wilson and Menkes disease proteins. The genome of the hyperthermophile Archaeoglobus fulgidus encodes a putative CopZ copper chaperone that contains an unusual cysteine-rich N-terminal domain of 130 amino acids in addition to a C-terminal copper binding domain with a conserved CXXC motif. The N-terminal domain (CopZ-NT) is homologous to proteins found only in extremophiles and is the only such protein that is fused to a copper chaperone. Surprisingly, optical, electron paramagnetic resonance, and x-ray absorption spectroscopic data indicate the presence of a [2Fe-2S] cluster in CopZ-NT. The intact CopZ protein binds two copper ions, one in each domain. The 1.8 A resolution crystal structure of CopZ-NT reveals that the [2Fe-2S] cluster is housed within a novel fold and that the protein also binds a zinc ion at a four-cysteine site. CopZ can deliver Cu+ to the A. fulgidus CopA N-terminal metal binding domain and is capable of reducing Cu2+ to Cu+. This unique fusion of a redox-active domain with a CXXC-containing copper chaperone domain is relevant to the evolution of copper homeostatic mechanisms and suggests new models for copper trafficking.
About this Structure
2HU9 is a Single protein structure of sequence from Archaeoglobus fulgidus. Full crystallographic information is available from OCA.
Reference
Characterization and structure of a Zn2+ and [2Fe-2S]-containing copper chaperone from Archaeoglobus fulgidus., Sazinsky MH, LeMoine B, Orofino M, Davydov R, Bencze KZ, Stemmler TL, Hoffman BM, Arguello JM, Rosenzweig AC, J Biol Chem. 2007 Aug 31;282(35):25950-9. Epub 2007 Jul 3. PMID:17609202 Page seeded by OCA on Sun May 4 06:43:02 2008
