User:Jennifer Taylor/Sandbox 4

4Q7Q is a ---- protein found in Chitinophaga pinensis, a soil bacterium in the sphingobacterial family. Its structure has been previously characterized and exists in Protein Data Bank. Its function, however, has not.

After structural and sequential analysis via various databases including BLAST, Pfam, Dali, PyMOL, and ProMOL, we initially predicted that 4Q7Q is a hydrolase. More specifically, we hypothesized that it was a lipase, an enzyme that can hydrolyze lipids to form fatty acids and a glycerol molecule.

In silico Analysis

We initially analyzed 4Q7Q through the protein structure databases BLAST, Pfam, and Dali. Our top hits were other known ----, including -----. Since we can use the principle of homology to predict the function of an unknown protein, we first hypothesized that 4Q7Q was too a ---. However, upon further analysis in ProMOL which involved the homology of active sites....

Bacterial Transformation

Protein Expression

Protein Purification

pNPB Lipase Assay

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