6b0c

From Proteopedia

(Difference between revisions)

Revision as of 10:09, 1 January 2020

KLP10A-AMPPNP in complex with curved tubulin and a microtubule

Structural highlights

6b0c is a 5 chain structure with sequence from Drome and Sus scrofa. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:	, , , ,
Gene:	Klp10A, CG1453 (DROME)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[TBA1B_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain. [KI10A_DROME] Required during anaphase to drive sister chromatid separation to promote flux by actively depolymerizing kinetochore microtubules at their pole-associated minus ends, thereby moving chromatids through a "poleward flux".^[1] [F2Z5B2_PIG] Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain.[RuleBase:RU003505]

Publication Abstract from PubMed

Kinesin-13s constitute a distinct group within the kinesin superfamily of motor proteins that promote microtubule depolymerization and lack motile activity. The molecular mechanism by which kinesin-13s depolymerize microtubules and are adapted to perform a seemingly very different activity from other kinesins is still unclear. To address this issue, here we report the near atomic resolution cryo-electron microscopy (cryo-EM) structures of Drosophila melanogaster kinesin-13 KLP10A protein constructs bound to curved or straight tubulin in different nucleotide states. These structures show how nucleotide induced conformational changes near the catalytic site are coupled with movement of the kinesin-13-specific loop-2 to induce tubulin curvature leading to microtubule depolymerization. The data highlight a modular structure that allows similar kinesin core motor-domains to be used for different functions, such as motility or microtubule depolymerization.

Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.,Benoit MPMH, Asenjo AB, Sosa H Nat Commun. 2018 Apr 25;9(1):1662. doi: 10.1038/s41467-018-04044-8. PMID:29695795^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Rogers GC, Rogers SL, Schwimmer TA, Ems-McClung SC, Walczak CE, Vale RD, Scholey JM, Sharp DJ. Two mitotic kinesins cooperate to drive sister chromatid separation during anaphase. Nature. 2004 Jan 22;427(6972):364-70. Epub 2003 Dec 14. PMID:14681690 doi:10.1038/nature02256
↑ Benoit MPMH, Asenjo AB, Sosa H. Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s. Nat Commun. 2018 Apr 25;9(1):1662. doi: 10.1038/s41467-018-04044-8. PMID:29695795 doi:http://dx.doi.org/10.1038/s41467-018-04044-8

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6b0c"

@@ Line 1: / Line 1: @@
 ==KLP10A-AMPPNP in complex with curved tubulin and a microtubule==
-<StructureSection load='6b0c' size='340' side='right' caption='[[6b0c]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
+<StructureSection load='6b0c' size='340' side='right'caption='[[6b0c]], [[Resolution|resolution]] 3.51&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6b0c]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/ ] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B0C FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6b0c]] is a 5 chain structure with sequence from [http://en.wikipedia.org/wiki/Drome Drome] and [http://en.wikipedia.org/wiki/Sus_scrofa Sus scrofa]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6B0C OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6B0C FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=GDP:GUANOSINE-5-DIPHOSPHATE'>GDP</scene>, <scene name='pdbligand=GTP:GUANOSINE-5-TRIPHOSPHATE'>GTP</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=TA1:TAXOL'>TA1</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Klp10A, CG1453 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=7227 DROME])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6b0c FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6b0c OCA], [http://pdbe.org/6b0c PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6b0c RCSB], [http://www.ebi.ac.uk/pdbsum/6b0c PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6b0c ProSAT]</span></td></tr>
 </table>
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 6b0c" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Tubulin|Tubulin]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Drome]]
+[[Category: Large Structures]]
 [[Category: Sus scrofa]]
 [[Category: Asenjo, A B]]

6b0c

From Proteopedia

Revision as of 10:09, 1 January 2020

KLP10A-AMPPNP in complex with curved tubulin and a microtubule

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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