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2a3g

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Revision as of 05:11, 13 February 2020

The structure of T6 bovine insulin

Structural highlights

2a3g is a 4 chain structure with sequence from Bos taurus. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Related:	4ins, 1mso, 1os3, 1os4
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[INS_BOVIN] Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

Publication Abstract from PubMed

Porcine insulin differs in sequence from bovine insulin at residues A8 (Thr in porcine-->Ala in bovine) and A10 (Ile in porcine-->Val in bovine). The structure of T6 hexameric bovine insulin has been determined to 2.25 A resolution at room temperature and refined to a residual of 0.162. The structure of the independent dimer is nearly identical to the T6 porcine insulin dimer: the mean displacement of all backbone atoms is 0.16 A, with the largest displacements occurring at AlaB30. Each of two independent zinc ions is octahedrally coordinated by three HisB10 side chains and three water molecules. As has been observed in both human and porcine insulin, the GluB13 side chains are directed towards the center of the hexamer, where a short contact of 2.57 A occurs between two independent carboxyl O atoms, again suggesting the presence of a centered hydrogen bond. No significant displacements of backbone atoms or changes in conformation are observed at A8 or A10. Since there are no interhexamer hydrogen-bonded contacts involving A8 in either porcine or bovine insulin, the change in the identity of this residue appears to have little or no effect upon the packing of the hexamers in the unit cell. In contrast, the side chains of the three A10 residues in one trimer make van der Waals contacts with the A10 side chains in a translationally related hexamer. As a consequence of the loss of the C(delta1) atom from the isoleucine residue in porcine insulin to produce valine in bovine insulin, there is a 0.36 A decrease in the distance between independent pairs of C(beta) atoms and a 0.24 A decrease in the c dimension of the unit cell. Thus, the net effect of the change in sequence at A10 is to strengthen the stabilizing hydrophobic interactions between hexamers.

The structure of T6 bovine insulin.,Smith GD, Pangborn WA, Blessing RH Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1476-82. Epub 2005, Oct 19. PMID:16239724^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Smith GD, Pangborn WA, Blessing RH. The structure of T6 bovine insulin. Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1476-82. Epub 2005, Oct 19. PMID:16239724 doi:10.1107/S0907444905025771

1 Structural highlights
2 Function
3 Evolutionary Conservation
4 Publication Abstract from PubMed
5 See Also
6 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2a3g"

@@ Line 1: / Line 1: @@
 ==The structure of T6 bovine insulin==
-<StructureSection load='2a3g' size='340' side='right' caption='[[2a3g]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
+<StructureSection load='2a3g' size='340' side='right'caption='[[2a3g]], [[Resolution|resolution]] 2.25&Aring;' scene=''>
 == Structural highlights ==
 <table><tr><td colspan='2'>[[2a3g]] is a 4 chain structure with sequence from [http://en.wikipedia.org/wiki/Bos_taurus Bos taurus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2A3G OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2A3G FirstGlance]. <br>
@@ Line 31: / Line 31: @@
 ==See Also==
-*[[Molecular Playground/Insulin|Molecular Playground/Insulin]]
+*[[Insulin 3D Structures|Insulin 3D Structures]]
 == References ==
 <references/>
@@ Line 37: / Line 37: @@
 </StructureSection>
 [[Category: Bos taurus]]
+[[Category: Large Structures]]
 [[Category: Blessing, R H]]
 [[Category: Pangborn, W A]]

2a3g

From Proteopedia

Revision as of 05:11, 13 February 2020

The structure of T6 bovine insulin

Structural highlights

Function

Evolutionary Conservation

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

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