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2i13
From Proteopedia
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[[Image:2i13.gif|left|200px]] | [[Image:2i13.gif|left|200px]] | ||
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'''Aart, a six finger zinc finger designed to recognize ANN triplets''' | '''Aart, a six finger zinc finger designed to recognize ANN triplets''' | ||
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[[Category: III, C F.Barbas.]] | [[Category: III, C F.Barbas.]] | ||
[[Category: Segal, D J.]] | [[Category: Segal, D J.]] | ||
| - | [[Category: | + | [[Category: Dna binding]] |
| - | [[Category: | + | [[Category: Zinc finger]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:56:51 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 03:56, 4 May 2008
Aart, a six finger zinc finger designed to recognize ANN triplets
Overview
Cys2-His2 zinc fingers are one of the most common types of DNA-binding domains. Modifications to zinc-finger binding specificity have recently enabled custom DNA-binding proteins to be designed to a wide array of target sequences. We present here a 1.96 A structure of Aart, a designed six-zinc finger protein, bound to a consensus DNA target site. This is the first structure of a designed protein with six fingers, and was intended to provide insights into the unusual affinity and specificity characteristics of this protein. Most protein-DNA contacts were found to be consistent with expectations, while others were unanticipated or insufficient to explain specificity. Several were unexpectedly mediated by glycerol, water molecules or amino acid-base stacking interactions. These results challenge some conventional concepts of recognition, particularly the finding that triplets containing 5'A, C, or T are typically not specified by direct interaction with the amino acid in position 6 of the recognition helix.
About this Structure
2I13 is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structure of Aart, a designed six-finger zinc finger peptide, bound to DNA., Segal DJ, Crotty JW, Bhakta MS, Barbas CF 3rd, Horton NC, J Mol Biol. 2006 Oct 20;363(2):405-21. Epub 2006 Aug 11. PMID:16963084 Page seeded by OCA on Sun May 4 06:56:51 2008
