2i1q

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[[Image:2i1q.jpg|left|200px]]
[[Image:2i1q.jpg|left|200px]]
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{{Structure
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<!--
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|PDB= 2i1q |SIZE=350|CAPTION= <scene name='initialview01'>2i1q</scene>, resolution 1.9&Aring;
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The line below this paragraph, containing "STRUCTURE_2i1q", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=ANP:PHOSPHOAMINOPHOSPHONIC+ACID-ADENYLATE+ESTER'>ANP</scene>, <scene name='pdbligand=CA:CALCIUM+ION'>CA</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY=
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or leave the SCENE parameter empty for the default display.
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|GENE= RADA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=2188 Methanococcus voltae])
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-->
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|DOMAIN=
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{{STRUCTURE_2i1q| PDB=2i1q | SCENE= }}
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|RELATEDENTRY=[[1z4d|1Z4D]]
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2i1q FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2i1q OCA], [http://www.ebi.ac.uk/pdbsum/2i1q PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2i1q RCSB]</span>
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}}
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'''RadA Recombinase in complex with Calcium'''
'''RadA Recombinase in complex with Calcium'''
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[[Category: Ma, X.]]
[[Category: Ma, X.]]
[[Category: Qian, X.]]
[[Category: Qian, X.]]
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[[Category: atp complex]]
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[[Category: Atp complex]]
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[[Category: atpase]]
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[[Category: Atpase]]
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[[Category: calcium stimulation]]
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[[Category: Calcium stimulation]]
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[[Category: dmc1]]
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[[Category: Dmc1]]
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[[Category: rad51]]
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[[Category: Rad51]]
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[[Category: rada]]
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[[Category: Rada]]
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[[Category: reca]]
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[[Category: Reca]]
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[[Category: recombinase]]
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[[Category: Recombinase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 06:58:19 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:38:36 2008''
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Revision as of 03:58, 4 May 2008

Image:2i1q.jpg

Template:STRUCTURE 2i1q

RadA Recombinase in complex with Calcium


Overview

Archaeal RadA or Rad51 recombinases are close homologues of eukaryal Rad51 and DMC1. These and bacterial RecA orthologues play a key role in DNA repair by forming helical nucleoprotein filaments in which a hallmark strand exchange reaction between homologous DNA substrates occurs. Recent studies have discovered the stimulatory role by calcium on human and yeast recombinases. Here we report that the strand exchange activity but not the ATPase activity of an archaeal RadA/Rad51 recombinase from Methanococcus voltae (MvRadA) is also subject to calcium stimulation. Crystallized MvRadA filaments in the presence of CaCl(2) resemble that of the recently reported ATPase active form in the presence of an activating dose of KCl. At the ATPase center, one Ca(2+) ion takes the place of two K(+) ions in the K(+)-bound form. The terminal phosphate of the nonhydrolyzable ATP analogue is in a staggered conformation in the Ca(2+)-bound form. In comparison, an eclipsed conformation was seen in the K(+)-bound form. Despite the changes in the ATPase center, both forms harbor largely ordered L2 regions in essentially identical conformations. These data suggest a unified stimulation mechanism by potassium and calcium because of the existence of a conserved ATPase center promiscuous in binding cations.

About this Structure

2I1Q is a Single protein structure of sequence from Methanococcus voltae. Full crystallographic information is available from OCA.

Reference

Calcium stiffens archaeal Rad51 recombinase from Methanococcus voltae for homologous recombination., Qian X, He Y, Ma X, Fodje MN, Grochulski P, Luo Y, J Biol Chem. 2006 Dec 22;281(51):39380-7. Epub 2006 Oct 17. PMID:17050545 Page seeded by OCA on Sun May 4 06:58:19 2008

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OCA

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