6ch2

From Proteopedia

(Difference between revisions)

Revision as of 10:45, 1 January 2020

Crystal structure of the cytoplasmic domain of FlhA and FliT-FliD complex

Structural highlights

6ch2 is a 6 chain structure with sequence from "bacillus_typhi"_schroeter_1886 "bacillus typhi" schroeter 1886 and Salty. Full crystallographic information is available from OCA. For a guided tour on the structure components use FirstGlance.
Ligands:
Gene:	flhA, STM1913 (SALTY), fliT, STY2170, t0915 ("Bacillus typhi" Schroeter 1886)
Resources:	FirstGlance, OCA, PDBe, RCSB, PDBsum, ProSAT

Function

[FLHA_SALTY] Required for formation of the rod structure of the flagellar apparatus. Together with FliI and FliH, may constitute the export apparatus of flagellin. [FLID_SALTY] Required for the morphogenesis and for the elongation of the flagellar filament by facilitating polymerization of the flagellin monomers at the tip of growing filament. Forms a capping structure, which prevents flagellin subunits (transported through the central channel of the flagellum) from leaking out without polymerization at the distal end.

Publication Abstract from PubMed

The flagellum and the injectisome enable bacterial locomotion and pathogenesis, respectively. These nanomachines assemble and function using a type III secretion system (T3SS). Exported proteins are delivered to the export apparatus by dedicated cytoplasmic chaperones for their transport through the membrane. The structural and mechanistic basis of this process is poorly understood. Here we report the structures of two ternary complexes among flagellar chaperones (FliT and FliS), protein substrates (the filament-capping FliD and flagellin FliC), and the export gate platform protein FlhA. The substrates do not interact directly with FlhA; however, they are required to induce a binding-competent conformation to the chaperone that exposes the recognition motif featuring a highly conserved sequence recognized by FlhA. The structural data reveal the recognition signal in a class of T3SS proteins and provide new insight into the assembly of key protein complexes at the export gate.

Structures of chaperone-substrate complexes docked onto the export gate in a type III secretion system.,Xing Q, Shi K, Portaliou A, Rossi P, Economou A, Kalodimos CG Nat Commun. 2018 May 2;9(1):1773. doi: 10.1038/s41467-018-04137-4. PMID:29720631^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.

References

↑ Xing Q, Shi K, Portaliou A, Rossi P, Economou A, Kalodimos CG. Structures of chaperone-substrate complexes docked onto the export gate in a type III secretion system. Nat Commun. 2018 May 2;9(1):1773. doi: 10.1038/s41467-018-04137-4. PMID:29720631 doi:http://dx.doi.org/10.1038/s41467-018-04137-4

1 Structural highlights
2 Function
3 Publication Abstract from PubMed
4 See Also
5 References

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/6ch2"

@@ Line 1: / Line 1: @@
 ==Crystal structure of the cytoplasmic domain of FlhA and FliT-FliD complex==
-<StructureSection load='6ch2' size='340' side='right' caption='[[6ch2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
+<StructureSection load='6ch2' size='340' side='right'caption='[[6ch2]], [[Resolution|resolution]] 2.70&Aring;' scene=''>
 == Structural highlights ==
-<table><tr><td colspan='2'>[[6ch2]] is a 6 chain structure. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CH2 FirstGlance]. <br>
+<table><tr><td colspan='2'>[[6ch2]] is a 6 chain structure with sequence from [http://en.wikipedia.org/wiki/"bacillus_typhi"_schroeter_1886 "bacillus typhi" schroeter 1886] and [http://en.wikipedia.org/wiki/Salty Salty]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=6CH2 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6CH2 FirstGlance]. <br>
 </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=GOL:GLYCEROL'>GOL</scene></td></tr>
+<tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">flhA, STM1913 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=99287 SALTY]), fliT, STY2170, t0915 ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=90370 "Bacillus typhi" Schroeter 1886])</td></tr>
 <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=6ch2 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=6ch2 OCA], [http://pdbe.org/6ch2 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=6ch2 RCSB], [http://www.ebi.ac.uk/pdbsum/6ch2 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=6ch2 ProSAT]</span></td></tr>
 </table>
@@ Line 18: / Line 19: @@
 </div>
 <div class="pdbe-citations 6ch2" style="background-color:#fffaf0;"></div>
+==See Also==
+*[[Flagellar protein 3D structures|Flagellar protein 3D structures]]
 == References ==
 <references/>
 __TOC__
 </StructureSection>
+[[Category: Bacillus typhi schroeter 1886]]
+[[Category: Large Structures]]
+[[Category: Salty]]
 [[Category: Kalodimos, C G]]
 [[Category: Shi, K]]

6ch2

From Proteopedia

Revision as of 10:45, 1 January 2020

Crystal structure of the cytoplasmic domain of FlhA and FliT-FliD complex

Structural highlights

Function

Publication Abstract from PubMed

See Also

References

Contents

Proteopedia Page Contributors and Editors (what is this?)

Views

Personal tools

Navigation

Search

Toolbox