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| | ==Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to TG100-115== | | ==Crystal structure of murine receptor-interacting protein 4 (Ripk4) D143N bound to TG100-115== |
| - | <StructureSection load='5wnk' size='340' side='right' caption='[[5wnk]], [[Resolution|resolution]] 3.11Å' scene=''> | + | <StructureSection load='5wnk' size='340' side='right'caption='[[5wnk]], [[Resolution|resolution]] 3.11Å' scene=''> |
| | == Structural highlights == | | == Structural highlights == |
| - | <table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure with sequence from [http://en.wikipedia.org/wiki/Lk3_transgenic_mice Lk3 transgenic mice]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5WNK FirstGlance]. <br> | + | <table><tr><td colspan='2'>[[5wnk]] is a 1 chain structure with sequence from [https://en.wikipedia.org/wiki/Mus_musculus Mus musculus]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5WNK OCA]. For a <b>guided tour on the structure components</b> use [https://proteopedia.org/fgij/fg.htm?mol=5WNK FirstGlance]. <br> |
| - | </td></tr><tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat"><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> | + | </td></tr><tr id='method'><td class="sblockLbl"><b>[[Empirical_models|Method:]]</b></td><td class="sblockDat" id="methodDat">X-ray diffraction, [[Resolution|Resolution]] 3.11Å</td></tr> |
| - | <tr id='related'><td class="sblockLbl"><b>[[Related_structure|Related:]]</b></td><td class="sblockDat">[[5wni|5wni]], [[5wnj|5wnj]], [[5wnl|5wnl]], [[5wnm|5wnm]]</td></tr>
| + | <tr id='ligand'><td class="sblockLbl"><b>[[Ligand|Ligands:]]</b></td><td class="sblockDat" id="ligandDat"><scene name='pdbligand=B6J:3,3-(2,4-diaminopteridine-6,7-diyl)diphenol'>B6J</scene>, <scene name='pdbligand=CL:CHLORIDE+ION'>CL</scene></td></tr> |
| - | <tr id='gene'><td class="sblockLbl"><b>[[Gene|Gene:]]</b></td><td class="sblockDat">Ripk4, Ankrd3, Pkk ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 LK3 transgenic mice])</td></tr> | + | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[https://proteopedia.org/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [https://pdbe.org/5wnk PDBe], [https://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [https://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [https://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr> |
| - | <tr id='activity'><td class="sblockLbl"><b>Activity:</b></td><td class="sblockDat"><span class='plainlinks'>[http://en.wikipedia.org/wiki/Non-specific_serine/threonine_protein_kinase Non-specific serine/threonine protein kinase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.11.1 2.7.11.1] </span></td></tr>
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| - | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5wnk FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5wnk OCA], [http://pdbe.org/5wnk PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5wnk RCSB], [http://www.ebi.ac.uk/pdbsum/5wnk PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5wnk ProSAT]</span></td></tr> | + | |
| | </table> | | </table> |
| | == Function == | | == Function == |
| - | [[http://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE]] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref> | + | [https://www.uniprot.org/uniprot/RIPK4_MOUSE RIPK4_MOUSE] Involved in stratified epithelial development (By similarity). It is a direct transcriptional target of TP63. Plays a role in NF-kappa-B activation.<ref>PMID:12446564</ref> <ref>PMID:22197488</ref> |
| - | <div style="background-color:#fffaf0;">
| + | |
| - | == Publication Abstract from PubMed ==
| + | |
| - | Receptor-interacting protein kinase 4 (RIPK4) is a highly conserved regulator of epidermal differentiation. Members of the RIPK family possess a common kinase domain as well as unique accessory domains that likely dictate subcellular localization and substrate preferences. Mutations in human RIPK4 manifest as Bartsocas-Papas syndrome (BPS), a genetic disorder characterized by severe craniofacial and limb abnormalities. We describe the structure of the murine Ripk4 (MmRipk4) kinase domain, in ATP- and inhibitor-bound forms. The crystallographic dimer of MmRipk4 is similar to those of RIPK2 and BRAF, and we show that the intact dimeric entity is required for MmRipk4 catalytic activity through a series of engineered mutations and cell-based assays. We also assess the impact of BPS mutations on protein structure and activity to elucidate the molecular origins of the disease.
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| - | Crystal Structure of Ripk4 Reveals Dimerization-Dependent Kinase Activity.,Huang CS, Oberbeck N, Hsiao YC, Liu P, Johnson AR, Dixit VM, Hymowitz SG Structure. 2018 May 1;26(5):767-777.e5. doi: 10.1016/j.str.2018.04.002. Epub 2018, Apr 26. PMID:29706531<ref>PMID:29706531</ref>
| + | ==See Also== |
| - | | + | *[[Ankyrin repeat domain-containing protein 3D structures|Ankyrin repeat domain-containing protein 3D structures]] |
| - | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br>
| + | *[[Serine/threonine protein kinase 3D structures|Serine/threonine protein kinase 3D structures]] |
| - | </div>
| + | |
| - | <div class="pdbe-citations 5wnk" style="background-color:#fffaf0;"></div>
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| | == References == | | == References == |
| | <references/> | | <references/> |
| | __TOC__ | | __TOC__ |
| | </StructureSection> | | </StructureSection> |
| - | [[Category: Lk3 transgenic mice]] | + | [[Category: Large Structures]] |
| - | [[Category: Non-specific serine/threonine protein kinase]] | + | [[Category: Mus musculus]] |
| - | [[Category: Huang, C S]] | + | [[Category: Huang CS]] |
| - | [[Category: Hymowitz, S G]] | + | [[Category: Hymowitz SG]] |
| - | [[Category: Complex]]
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| - | [[Category: Inhibitor]]
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| - | [[Category: Kinase]]
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| - | [[Category: Transferase]]
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| - | [[Category: Transferase-transferase inhibitor complex]]
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