This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
5yo8
From Proteopedia
(Difference between revisions)
| Line 1: | Line 1: | ||
==Crystal structure of beta-C25/C30/C35-prene synthase== | ==Crystal structure of beta-C25/C30/C35-prene synthase== | ||
| - | <StructureSection load='5yo8' size='340' side='right' caption='[[5yo8]], [[Resolution|resolution]] 1.64Å' scene=''> | + | <StructureSection load='5yo8' size='340' side='right'caption='[[5yo8]], [[Resolution|resolution]] 1.64Å' scene=''> |
== Structural highlights == | == Structural highlights == | ||
<table><tr><td colspan='2'>[[5yo8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_alcalophilus_atcc_27647 Bacillus alcalophilus atcc 27647]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YO8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YO8 FirstGlance]. <br> | <table><tr><td colspan='2'>[[5yo8]] is a 2 chain structure with sequence from [http://en.wikipedia.org/wiki/Bacillus_alcalophilus_atcc_27647 Bacillus alcalophilus atcc 27647]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=5YO8 OCA]. For a <b>guided tour on the structure components</b> use [http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5YO8 FirstGlance]. <br> | ||
| Line 7: | Line 7: | ||
<tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yo8 OCA], [http://pdbe.org/5yo8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yo8 RCSB], [http://www.ebi.ac.uk/pdbsum/5yo8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yo8 ProSAT]</span></td></tr> | <tr id='resources'><td class="sblockLbl"><b>Resources:</b></td><td class="sblockDat"><span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=5yo8 FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=5yo8 OCA], [http://pdbe.org/5yo8 PDBe], [http://www.rcsb.org/pdb/explore.do?structureId=5yo8 RCSB], [http://www.ebi.ac.uk/pdbsum/5yo8 PDBsum], [http://prosat.h-its.org/prosat/prosatexe?pdbcode=5yo8 ProSAT]</span></td></tr> | ||
</table> | </table> | ||
| + | <div style="background-color:#fffaf0;"> | ||
| + | == Publication Abstract from PubMed == | ||
| + | Thousands of terpenes have been identified to date. However, only two classes of enzymes are known to be involved in their biosynthesis, and each class has characteristic amino-acid motifs. We recently identified a novel large-terpene (C25/C30/C35) synthase, which shares no motifs with known enzymes. To elucidate the molecular mechanism of this enzyme, we determined the crystal structure of a large-beta-prene synthase from B. alcalophilus (BalTS). Surprisingly, the overall structure of BalTS is similar to that of the alpha-domain of class I terpene synthases although their primary structures are totally different from each other. Two novel aspartate-rich motifs, DYLDNLxD and DY(F,L,W)IDxxED, are identified, and mutations of any one of the aspartates eliminate its enzymatic activity. The present work leads us to propose a new subclass of terpene synthases, class IB, which is probably responsible for large-terpene biosynthesis. | ||
| + | |||
| + | Crystal structure and functional analysis of large-terpene synthases belonging to a newly found subclass.,Fujihashi M, Sato T, Tanaka Y, Yamamoto D, Nishi T, Ueda D, Murakami M, Yasuno Y, Sekihara A, Fuku K, Shinada T, Miki K Chem Sci. 2018 Mar 16;9(15):3754-3758. doi: 10.1039/c8sc00289d. eCollection 2018 , Apr 21. PMID:29780507<ref>PMID:29780507</ref> | ||
| + | |||
| + | From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.<br> | ||
| + | </div> | ||
| + | <div class="pdbe-citations 5yo8" style="background-color:#fffaf0;"></div> | ||
| + | == References == | ||
| + | <references/> | ||
__TOC__ | __TOC__ | ||
</StructureSection> | </StructureSection> | ||
[[Category: Bacillus alcalophilus atcc 27647]] | [[Category: Bacillus alcalophilus atcc 27647]] | ||
| + | [[Category: Large Structures]] | ||
[[Category: Fujihashi, M]] | [[Category: Fujihashi, M]] | ||
[[Category: Miki, K]] | [[Category: Miki, K]] | ||
[[Category: Lyase]] | [[Category: Lyase]] | ||
[[Category: Terpene synthase]] | [[Category: Terpene synthase]] | ||
Revision as of 12:43, 25 December 2019
Crystal structure of beta-C25/C30/C35-prene synthase
| |||||||||||
