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2i7t
From Proteopedia
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'''Structure of human CPSF-73''' | '''Structure of human CPSF-73''' | ||
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[[Category: Tong, L.]] | [[Category: Tong, L.]] | ||
[[Category: Zhang, H.]] | [[Category: Zhang, H.]] | ||
| - | [[Category: | + | [[Category: Artemi]] |
| - | [[Category: | + | [[Category: Double-strand break repair]] |
| - | [[Category: | + | [[Category: Metallo-b-lactamase]] |
| - | [[Category: | + | [[Category: Polyadenylation]] |
| - | [[Category: | + | [[Category: Pre-mrna processing]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:11:09 2008'' | |
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Revision as of 04:11, 4 May 2008
Structure of human CPSF-73
Overview
Most eukaryotic messenger RNA precursors (pre-mRNAs) undergo extensive maturational processing, including cleavage and polyadenylation at the 3'-end. Despite the characterization of many proteins that are required for the cleavage reaction, the identity of the endonuclease is not known. Recent analyses indicated that the 73-kDa subunit of cleavage and polyadenylation specificity factor (CPSF-73) might be the endonuclease for this and related reactions, although no direct data confirmed this. Here we report the crystal structures of human CPSF-73 at 2.1 A resolution, complexed with zinc ions and a sulphate that might mimic the phosphate group of the substrate, and the related yeast protein CPSF-100 (Ydh1) at 2.5 A resolution. Both CPSF-73 and CPSF-100 contain two domains, a metallo-beta-lactamase domain and a novel beta-CASP (named for metallo-beta-lactamase, CPSF, Artemis, Snm1, Pso2) domain. The active site of CPSF-73, with two zinc ions, is located at the interface of the two domains. Purified recombinant CPSF-73 possesses RNA endonuclease activity, and mutations that disrupt zinc binding in the active site abolish this activity. Our studies provide the first direct experimental evidence that CPSF-73 is the pre-mRNA 3'-end-processing endonuclease.
About this Structure
2I7T is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Polyadenylation factor CPSF-73 is the pre-mRNA 3'-end-processing endonuclease., Mandel CR, Kaneko S, Zhang H, Gebauer D, Vethantham V, Manley JL, Tong L, Nature. 2006 Dec 14;444(7121):953-6. Epub 2006 Nov 26. PMID:17128255 Page seeded by OCA on Sun May 4 07:11:09 2008
