2ido

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[[Image:2ido.gif|left|200px]]
[[Image:2ido.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ido |SIZE=350|CAPTION= <scene name='initialview01'>2ido</scene>, resolution 2.10&Aring;
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The line below this paragraph, containing "STRUCTURE_2ido", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EDO:1,2-ETHANEDIOL'>EDO</scene>, <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=TMP:THYMIDINE-5&#39;-PHOSPHATE'>TMP</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= dnaQ, mutD ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=562 Escherichia coli]), hot ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10678 Enterobacteria phage P1])
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-->
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|DOMAIN=
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{{STRUCTURE_2ido| PDB=2ido | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ido FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ido OCA], [http://www.ebi.ac.uk/pdbsum/2ido PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ido RCSB]</span>
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}}
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'''Structure of the E. coli Pol III epsilon-Hot proofreading complex'''
'''Structure of the E. coli Pol III epsilon-Hot proofreading complex'''
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[[Category: Perrino, F W.]]
[[Category: Perrino, F W.]]
[[Category: Schaaper, R M.]]
[[Category: Schaaper, R M.]]
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[[Category: epsilon]]
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[[Category: Epsilon]]
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[[Category: exonuclease]]
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[[Category: Exonuclease]]
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[[Category: hot]]
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[[Category: Hot]]
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[[Category: pol iii]]
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[[Category: Pol iii]]
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[[Category: polymerase]]
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[[Category: Polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:22:44 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:42:52 2008''
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Revision as of 04:22, 4 May 2008

Image:2ido.gif

Template:STRUCTURE 2ido

Structure of the E. coli Pol III epsilon-Hot proofreading complex


Overview

The epsilon subunit of Escherichia coli DNA polymerase III possesses 3'-exonucleolytic proofreading activity. Within the Pol III core, epsilon is tightly bound between the alpha subunit (DNA polymerase) and subunit. Here, we present the crystal structure of epsilon in complex with HOT, the bacteriophage P1-encoded homolog of , at 2.1 A resolution. The epsilon-HOT interface is defined by two areas of contact: an interaction of the previously unstructured N terminus of HOT with an edge of the epsilon central beta-sheet as well as interactions between HOT and the catalytically important helix alpha1-loop-helix alpha2 motif of epsilon. This structure provides insight into how HOT and, by implication, may stabilize the epsilon subunit, thus promoting efficient proofreading during chromosomal replication.

About this Structure

2IDO is a Protein complex structure of sequences from Enterobacteria phage p1 and Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of the Escherichia coli DNA polymerase III epsilon-HOT proofreading complex., Kirby TW, Harvey S, DeRose EF, Chalov S, Chikova AK, Perrino FW, Schaaper RM, London RE, Pedersen LC, J Biol Chem. 2006 Dec 15;281(50):38466-71. Epub 2006 Sep 13. PMID:16973612 Page seeded by OCA on Sun May 4 07:22:44 2008

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