2iff

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[[Image:2iff.gif|left|200px]]
[[Image:2iff.gif|left|200px]]
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{{Structure
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The line below this paragraph, containing "STRUCTURE_2iff", creates the "Structure Box" on the page.
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{{STRUCTURE_2iff| PDB=2iff | SCENE= }}
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2iff FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2iff OCA], [http://www.ebi.ac.uk/pdbsum/2iff PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2iff RCSB]</span>
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'''STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION'''
'''STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION'''
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[[Category: Chacko, S.]]
[[Category: Chacko, S.]]
[[Category: Davies, D R.]]
[[Category: Davies, D R.]]
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[[Category: immunoglobulin/hydrolase(o-glycosyl)]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:26:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:43:36 2008''
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Revision as of 04:26, 4 May 2008

Image:2iff.gif

Template:STRUCTURE 2iff

STRUCTURE OF AN ANTIBODY-LYSOZYME COMPLEX: EFFECT OF A CONSERVATIVE MUTATION


Overview

The structure of the complex between the Fab HyHEL-5 and chicken lysozyme revealed a large interface region containing 23 lysozyme and 28 Fab residues. Arg68 of the lysozyme is centrally placed in this interface and theoretical studies together with binding assays of this Fab to different avian lysozymes have previously shown that this arginine residue is an important contributor to the binding. The Arg68-->Lys mutant binds 10(3) times less well to the HyHEL-5 Fab. We have examined the refined crystal structure of the complex of this mutant lysozyme with the Fab. No global changes occur, but there is an introduction of a new water molecule into the interface that mediates the hydrogen bonding interactions between the lysine and residues on the Fab. These data are compared with the effects of similar changes on the inhibition of serine proteases such as trypsin where the energetic effects of this substitution are small.

About this Structure

2IFF is a Protein complex structure of sequences from Gallus gallus and Mus musculus. Full crystallographic information is available from OCA.

Reference

Structure of an antibody-lysozyme complex unexpected effect of conservative mutation., Chacko S, Silverton E, Kam-Morgan L, Smith-Gill S, Cohen G, Davies D, J Mol Biol. 1995 Jan 20;245(3):261-74. PMID:7531245 Page seeded by OCA on Sun May 4 07:26:46 2008

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