2ig0
From Proteopedia
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[[Image:2ig0.jpg|left|200px]] | [[Image:2ig0.jpg|left|200px]] | ||
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'''Structure of 53BP1/methylated histone peptide complex''' | '''Structure of 53BP1/methylated histone peptide complex''' | ||
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[[Category: Single protein]] | [[Category: Single protein]] | ||
[[Category: Mer, G.]] | [[Category: Mer, G.]] | ||
| - | [[Category: | + | [[Category: Cell cycle regulation]] |
| - | [[Category: | + | [[Category: Dimethylated histone h4]] |
| - | [[Category: | + | [[Category: Dna repair]] |
| - | [[Category: | + | [[Category: Tandem tudor domain]] |
| - | + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:27:45 2008'' | |
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
Revision as of 04:27, 4 May 2008
Structure of 53BP1/methylated histone peptide complex
Overview
Histone lysine methylation has been linked to the recruitment of mammalian DNA repair factor 53BP1 and putative fission yeast homolog Crb2 to DNA double-strand breaks (DSBs), but how histone recognition is achieved has not been established. Here we demonstrate that this link occurs through direct binding of 53BP1 and Crb2 to histone H4. Using X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy, we show that, despite low amino acid sequence conservation, both 53BP1 and Crb2 contain tandem tudor domains that interact with histone H4 specifically dimethylated at Lys20 (H4-K20me2). The structure of 53BP1/H4-K20me2 complex uncovers a unique five-residue 53BP1 binding cage, remarkably conserved in the structure of Crb2, that best accommodates a dimethyllysine but excludes a trimethyllysine, thus explaining the methylation state-specific recognition of H4-K20. This study reveals an evolutionarily conserved molecular mechanism of targeting DNA repair proteins to DSBs by direct recognition of H4-K20me2.
About this Structure
2IG0 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structural basis for the methylation state-specific recognition of histone H4-K20 by 53BP1 and Crb2 in DNA repair., Botuyan MV, Lee J, Ward IM, Kim JE, Thompson JR, Chen J, Mer G, Cell. 2006 Dec 29;127(7):1361-73. PMID:17190600 Page seeded by OCA on Sun May 4 07:27:45 2008
