2ihm

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[[Image:2ihm.gif|left|200px]]
[[Image:2ihm.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ihm |SIZE=350|CAPTION= <scene name='initialview01'>2ihm</scene>, resolution 2.4&Aring;
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The line below this paragraph, containing "STRUCTURE_2ihm", creates the "Structure Box" on the page.
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|SITE=
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=D3T:2&#39;,3&#39;-DIDEOXY-THYMIDINE-5&#39;-TRIPHOSPHATE'>D3T</scene>, <scene name='pdbligand=DA:2&#39;-DEOXYADENOSINE-5&#39;-MONOPHOSPHATE'>DA</scene>, <scene name='pdbligand=DC:2&#39;-DEOXYCYTIDINE-5&#39;-MONOPHOSPHATE'>DC</scene>, <scene name='pdbligand=DG:2&#39;-DEOXYGUANOSINE-5&#39;-MONOPHOSPHATE'>DG</scene>, <scene name='pdbligand=DT:THYMIDINE-5&#39;-MONOPHOSPHATE'>DT</scene>, <scene name='pdbligand=MG:MAGNESIUM+ION'>MG</scene>, <scene name='pdbligand=NA:SODIUM+ION'>NA</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/DNA-directed_DNA_polymerase DNA-directed DNA polymerase], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=2.7.7.7 2.7.7.7] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE= Polm ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=10090 Mus musculus])
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-->
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|DOMAIN=
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{{STRUCTURE_2ihm| PDB=2ihm | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ihm FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ihm OCA], [http://www.ebi.ac.uk/pdbsum/2ihm PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ihm RCSB]</span>
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}}
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'''Polymerase mu in ternary complex with gapped 11mer DNA duplex and bound incoming nucleotide'''
'''Polymerase mu in ternary complex with gapped 11mer DNA duplex and bound incoming nucleotide'''
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[[Category: Moon, A F.]]
[[Category: Moon, A F.]]
[[Category: Pedersen, L C]]
[[Category: Pedersen, L C]]
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[[Category: helix-turn-helix]]
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[[Category: Helix-turn-helix]]
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[[Category: polymerase]]
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[[Category: Polymerase]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:30:49 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:44:27 2008''
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Revision as of 04:30, 4 May 2008

Image:2ihm.gif

Template:STRUCTURE 2ihm

Polymerase mu in ternary complex with gapped 11mer DNA duplex and bound incoming nucleotide


Overview

DNA polymerase mu (Pol mu) is a family X enzyme with unique substrate specificity that contributes to its specialized role in nonhomologous DNA end joining (NHEJ). To investigate Pol mu's unusual substrate specificity, we describe the 2.4 A crystal structure of the polymerase domain of murine Pol mu bound to gapped DNA with a correct dNTP at the active site. This structure reveals substrate interactions with side chains in Pol mu that differ from other family X members. For example, a single amino acid substitution, H329A, has little effect on template-dependent synthesis by Pol mu from a paired primer terminus, but it reduces both template-independent and template-dependent synthesis during NHEJ of intermediates whose 3' ends lack complementary template strand nucleotides. These results provide insight into the substrate specificity and differing functions of four closely related mammalian family X DNA polymerases.

About this Structure

2IHM is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.

Reference

Structural insight into the substrate specificity of DNA Polymerase mu., Moon AF, Garcia-Diaz M, Bebenek K, Davis BJ, Zhong X, Ramsden DA, Kunkel TA, Pedersen LC, Nat Struct Mol Biol. 2007 Jan;14(1):45-53. Epub 2006 Dec 10. PMID:17159995 Page seeded by OCA on Sun May 4 07:30:49 2008

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OCA

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