2iij
From Proteopedia
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'''Structure of human Asf1a in complex with histone H3''' | '''Structure of human Asf1a in complex with histone H3''' | ||
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[[Category: Mann, C.]] | [[Category: Mann, C.]] | ||
[[Category: Ochsenbein, F.]] | [[Category: Ochsenbein, F.]] | ||
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Revision as of 04:32, 4 May 2008
Structure of human Asf1a in complex with histone H3
Overview
Asf1 is a histone chaperone that favors histone H3/H4 assembly and disassembly. We solved the structure of the conserved domain of human ASF1A in complex with the C-terminal helix of histone H3 using nuclear magnetic resonance spectroscopy. This structure is fully compatible with an association of ASF1 with the heterodimeric form of histones H3/H4. In our model, ASF1 substitutes for the second H3/H4 heterodimer that is normally found in heterotetrameric H3/H4 complexes. This result constitutes an essential step in the fundamental understanding of the mechanisms of nucleosome assembly by histone chaperones. Point mutations that perturb the Asf1/histone interface were designed from the structure. The decreased binding affinity of the Asf1-H3/H4 complex correlates with decreased levels of H3-K56 acetylation and phenotypic defects in vivo.
About this Structure
2IIJ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the histone chaperone ASF1 bound to the histone H3 C-terminal helix and functional insights., Agez M, Chen J, Guerois R, van Heijenoort C, Thuret JY, Mann C, Ochsenbein F, Structure. 2007 Feb;15(2):191-9. PMID:17292837 Page seeded by OCA on Sun May 4 07:32:51 2008
