Structural highlights
Function
[BARF1_EBVB9] Plays diverse functions in immunomodulation and oncogenicity, maybe by acting as a functional receptor for human CSF1. May inhibit interferon secretion from mononuclear cells. Exhibits oncogenic activity in vitro.[1] [2] [3]
Evolutionary Conservation
Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.
Publication Abstract from PubMed
The Epstein-Barr virus is a human gamma-herpesvirus that persistently infects more than 90% of the human population. It is associated with numerous epithelial cancers, principally undifferentiated nasopharyngeal carcinoma and gastric carcinoma. The BARF1 gene is expressed in a high proportion of these cancers. An oncogenic, mitogenic and immortalizing activity of the BARF1 protein has been shown. We solved the structure of the secreted BARF1 glycoprotein expressed in a human cell line by X-ray crystallography at a resolution of 2.3A. The BARF1 protein consists of two immunoglobulin (Ig)-like domains. The N-terminal domain belongs to the subfamily of variable domains whereas the C-terminal one is related to a constant Ig-domain. BARF1 shows an unusual hexamerisation involving two principal contacts, one between the C-terminal domains and one between the N-terminal domains. The C-terminal contact with an uncommonly large contact surface extends the beta-sandwich of the Ig-domain through the second molecule. The N-terminal contact involves Ig-domains with an unusual relative orientation but with a more classical contact surface with a size in the range of dimer interactions of Ig-domains. The structure of BARF1 is most closely related to CD80 or B7-1, a co-stimulatory molecule present on antigen presenting cells, from which BARF1 must have been derived during evolution. Still, domain orientation and oligomerization differ between BARF1 and CD80. It had been shown that BARF1 binds to hCSF-1, the human colony-stimulating factor 1, but this interaction has to be principally different from the one between CSF-1 and CSF-1 receptor.
Structure of the Epstein-Barr virus oncogene BARF1.,Tarbouriech N, Ruggiero F, de Turenne-Tessier M, Ooka T, Burmeister WP J Mol Biol. 2006 Jun 9;359(3):667-78. Epub 2006 Apr 18. PMID:16647084[4]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sall A, Caserta S, Jolicoeur P, Franqueville L, de Turenne-Tessier M, Ooka T. Mitogenic activity of Epstein-Barr virus-encoded BARF1 protein. Oncogene. 2004 Jun 17;23(28):4938-44. PMID:15064715 doi:10.1038/sj.onc.1207607
- ↑ Seto E, Yang L, Middeldorp J, Sheen TS, Chen JY, Fukayama M, Eizuru Y, Ooka T, Takada K. Epstein-Barr virus (EBV)-encoded BARF1 gene is expressed in nasopharyngeal carcinoma and EBV-associated gastric carcinoma tissues in the absence of lytic gene expression. J Med Virol. 2005 May;76(1):82-8. PMID:15778977 doi:10.1002/jmv.20327
- ↑ Wang Q, Tsao SW, Ooka T, Nicholls JM, Cheung HW, Fu S, Wong YC, Wang X. Anti-apoptotic role of BARF1 in gastric cancer cells. Cancer Lett. 2006 Jul 8;238(1):90-103. Epub 2005 Jul 27. PMID:16054293 doi:10.1016/j.canlet.2005.06.023
- ↑ Tarbouriech N, Ruggiero F, de Turenne-Tessier M, Ooka T, Burmeister WP. Structure of the Epstein-Barr virus oncogene BARF1. J Mol Biol. 2006 Jun 9;359(3):667-78. Epub 2006 Apr 18. PMID:16647084 doi:10.1016/j.jmb.2006.03.056
