2ind

From Proteopedia

(Difference between revisions)
Jump to: navigation, search
Line 1: Line 1:
[[Image:2ind.gif|left|200px]]
[[Image:2ind.gif|left|200px]]
-
{{Structure
+
<!--
-
|PDB= 2ind |SIZE=350|CAPTION= <scene name='initialview01'>2ind</scene>, resolution 2.200&Aring;
+
The line below this paragraph, containing "STRUCTURE_2ind", creates the "Structure Box" on the page.
-
|SITE=
+
You may change the PDB parameter (which sets the PDB file loaded into the applet)
-
|LIGAND= <scene name='pdbligand=MN:MANGANESE+(II)+ION'>MN</scene>, <scene name='pdbligand=P6G:HEXAETHYLENE+GLYCOL'>P6G</scene>
+
or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
-
|ACTIVITY=
+
or leave the SCENE parameter empty for the default display.
-
|GENE= touA ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), touE ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri]), touB ([http://www.ncbi.nlm.nih.gov/Taxonomy/Browser/wwwtax.cgi?mode=Info&srchmode=5&id=316 Pseudomonas stutzeri])
+
-->
-
|DOMAIN=
+
{{STRUCTURE_2ind| PDB=2ind | SCENE= }}
-
|RELATEDENTRY=[[1t0q|1T0Q]]
+
-
|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ind FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ind OCA], [http://www.ebi.ac.uk/pdbsum/2ind PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ind RCSB]</span>
+
-
}}
+
'''Mn(II) Reconstituted Toluene/o-xylene Monooxygenase Hydroxylase X-ray Crystal Structure'''
'''Mn(II) Reconstituted Toluene/o-xylene Monooxygenase Hydroxylase X-ray Crystal Structure'''
Line 30: Line 27:
[[Category: Sazinsky, M H.]]
[[Category: Sazinsky, M H.]]
[[Category: 4-helix bundle]]
[[Category: 4-helix bundle]]
-
[[Category: carboxylate bridge]]
+
[[Category: Carboxylate bridge]]
-
[[Category: manganese reconstitution]]
+
[[Category: Manganese reconstitution]]
-
[[Category: metalloenzyme]]
+
[[Category: Metalloenzyme]]
-
 
+
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 07:41:14 2008''
-
''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:46:26 2008''
+

Revision as of 04:41, 4 May 2008

Image:2ind.gif

Template:STRUCTURE 2ind

Mn(II) Reconstituted Toluene/o-xylene Monooxygenase Hydroxylase X-ray Crystal Structure


Overview

We report the X-ray crystal structures of native and manganese(II)-reconstituted toluene/o-xylene monooxygenase hydroxylase (ToMOH) from Pseudomonas stutzeri OX1 to 1.85 and 2.20 A resolution, respectively. The structures reveal that reduction of the dimetallic active site is accompanied by a carboxylate shift and alteration of the coordination environment for dioxygen binding and activation. A rotamer shift in a strategically placed asparagine 202 accompanies dimetallic center reduction and is proposed to influence protein component interactions. This rotamer shift is conserved between ToMOH and the corresponding residue in methane monooxygenase hydroxylase (MMOH). Previously unidentified hydrophobic pockets similar to those present in MMOH are assigned.

About this Structure

2IND is a Protein complex structure of sequences from Pseudomonas stutzeri. Full crystallographic information is available from OCA.

Reference

X-ray crystal structures of manganese(II)-reconstituted and native toluene/o-xylene monooxygenase hydroxylase reveal rotamer shifts in conserved residues and an enhanced view of the protein interior., McCormick MS, Sazinsky MH, Condon KL, Lippard SJ, J Am Chem Soc. 2006 Nov 29;128(47):15108-10. PMID:17117860 Page seeded by OCA on Sun May 4 07:41:14 2008

Proteopedia Page Contributors and Editors (what is this?)

OCA

Retrieved from "http://52.214.119.220/wiki/index.php/2ind"
Personal tools