2ixs

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[[Image:2ixs.gif|left|200px]]
[[Image:2ixs.gif|left|200px]]
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{{Structure
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<!--
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|PDB= 2ixs |SIZE=350|CAPTION= <scene name='initialview01'>2ixs</scene>, resolution 2.00&Aring;
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The line below this paragraph, containing "STRUCTURE_2ixs", creates the "Structure Box" on the page.
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|SITE= <scene name='pdbsite=AC1:Trs+Binding+Site+For+Chain+B'>AC1</scene>
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You may change the PDB parameter (which sets the PDB file loaded into the applet)
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|LIGAND= <scene name='pdbligand=EPE:4-(2-HYDROXYETHYL)-1-PIPERAZINE+ETHANESULFONIC+ACID'>EPE</scene>, <scene name='pdbligand=SO4:SULFATE+ION'>SO4</scene>, <scene name='pdbligand=TRS:2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL'>TRS</scene>
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or the SCENE parameter (which sets the initial scene displayed when the page is loaded),
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|ACTIVITY= <span class='plainlinks'>[http://en.wikipedia.org/wiki/Type_II_site-specific_deoxyribonuclease Type II site-specific deoxyribonuclease], with EC number [http://www.brenda-enzymes.info/php/result_flat.php4?ecno=3.1.21.4 3.1.21.4] </span>
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or leave the SCENE parameter empty for the default display.
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|GENE=
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-->
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|DOMAIN=
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{{STRUCTURE_2ixs| PDB=2ixs | SCENE= }}
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|RELATEDENTRY=
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|RESOURCES=<span class='plainlinks'>[http://oca.weizmann.ac.il/oca-docs/fgij/fg.htm?mol=2ixs FirstGlance], [http://oca.weizmann.ac.il/oca-bin/ocaids?id=2ixs OCA], [http://www.ebi.ac.uk/pdbsum/2ixs PDBsum], [http://www.rcsb.org/pdb/explore.do?structureId=2ixs RCSB]</span>
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}}
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'''STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE'''
'''STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE'''
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[[Category: Tamulaitiene, G.]]
[[Category: Tamulaitiene, G.]]
[[Category: Urbanke, C.]]
[[Category: Urbanke, C.]]
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[[Category: domain architecture]]
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[[Category: Domain architecture]]
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[[Category: endonuclease]]
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[[Category: Endonuclease]]
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[[Category: hydrolase]]
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[[Category: Hydrolase]]
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[[Category: restriction endonuclease]]
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[[Category: Restriction endonuclease]]
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[[Category: sdai]]
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[[Category: Sdai]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 08:03:05 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Mar 31 03:50:10 2008''
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Revision as of 05:03, 4 May 2008

Image:2ixs.gif

Template:STRUCTURE 2ixs

STRUCTURE OF SDAI RESTRICTION ENDONUCLEASE


Overview

Rare-cutting restriction enzymes are important tools in genome analysis. We report here the crystal structure of SdaI restriction endonuclease, which is specific for the 8 bp sequence CCTGCA/GG ("/" designates the cleavage site). Unlike orthodox Type IIP enzymes, which are single domain proteins, the SdaI monomer is composed of two structural domains. The N domain contains a classical winged helix-turn-helix (wHTH) DNA binding motif, while the C domain shows a typical restriction endonuclease fold. The active site of SdaI is located within the C domain and represents a variant of the canonical PD-(D/E)XK motif. SdaI determinants of sequence specificity are clustered on the recognition helix of the wHTH motif at the N domain. The modular architecture of SdaI, wherein one domain mediates DNA binding while the other domain is predicted to catalyze hydrolysis, distinguishes SdaI from previously characterized restriction enzymes interacting with symmetric recognition sequences.

About this Structure

2IXS is a Single protein structure of sequence from Streptomyces diastaticus. Full crystallographic information is available from OCA.

Reference

The crystal structure of the rare-cutting restriction enzyme SdaI reveals unexpected domain architecture., Tamulaitiene G, Jakubauskas A, Urbanke C, Huber R, Grazulis S, Siksnys V, Structure. 2006 Sep;14(9):1389-400. PMID:16962970 Page seeded by OCA on Sun May 4 08:03:05 2008

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OCA

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